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Dr Frank Menke

Head of Proteomics
Proteomics

I have been working on defence signal transduction and protein phosphorylation through out most of my professional life, progressing from single molecule analysis towards proteome scale networks.

My initial work focussed on elicitor triggered mitogen activated protein kinases (Menke et al, 2004; Menke et al, 2005). To address defence signalling in an unbiased way I set up quantitative shotgun phospho-proteomics using stabile isotope labelling in Arabidopsis which I also applied to lateral root initiation (Benschop et al, 2007; Mithoe and Menke 2011; Mithoe et al., 2012; Zhang et al 2013).

I’m currently developing stabile isotope based proteomics methods for network biology of early defence signalling based on targeted phospho-proteomics using selected reaction monitoring (collaboration with Prof Ruedi Aebersold). These quantitative proteomics approaches will help to decipher the signalling network required for microbe associated molecular pattern-triggered immunity in Arabidopsis.

Dr Frank Menke

frank.Menke@tsl.ac.uk
+44 (0) 1603 450225

Publications

Wirthmueller L, Asai S, Rallapalli G, Sklenar J, Fabro G, Kim DS, Lintermann R, Jaspers P, Wrzaczek M, Kangasjärvi L, MacLean D, Menke FLH, Banfield MJ, JDG (2017)

An Oomycete Effector Protein Induces Shade Avoidance In Arabidopsis And Attenuates Salicylate Signaling By Binding To Host Proteins Of The RADICAL-INDUCED CELL DEATH1 Family. bioRxiv preprint May 14, 2017 doi: https://doi.org/10.1101/137844

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Menke FLH (2016)

Plants get on PAR with poly(ADP‐ribosyl)ation. New & Views. EMBO reports (2016) e201643453. DOI 10.15252/embr.201643453

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Couto D, Niebergall R, Liang X, Bücherl CA, Sklenar J, Macho AP, Ntoukakis V, Derbyshire P, Altenbach D, Maclean D, Robatzek S, Uhrig J, Menke F, Zhou JM, Zipfel C (2016)

The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1. PLoS Pathog. 12(8):e1005811. doi: 10.1371/journal.ppat.1005811.

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Mithoe SC, Ludwig C, Pel MJC, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CMJ, Aebersold R, Menke FLH (2015)

Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO reports (2016). DOI 10.15252/embr.201540806

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Dagdas YF, Belhaj K, Maqbool A, Chaparro-Garcia A, Pandey P, Petre B, Tabassum N, Cruz-Mireles N, Hughes RK, Sklenar J, Win J, Menke F, Findlay K, Banfield MJ, Kamoun S, Bozkurt TO

An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor. Elife. 2016 Jan 14;5. pii: e10856. doi: 10.7554/eLife.10856

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Gupta YK, Dagdas YF, Martinez-Rochaa A-L, Kershaw MJ, Littlejohn GR, Rydera LS, Sklenar J, Menke F, Talbot NJ (2015)

Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by Magnaporthe oryzae. The Plant Cell doi: http:/​/​dx.​doi.​org/​10.​1105/​tpc.​15.​00552

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Sarris PF, Duxbury Z, Huh SU, Yan Ma, Segonzac C, Sklenar J, Derbyshire P, Cevik V, Rallapalli G, Saucet SB, Wirthmueller L, Menke FLH, Sohn KH, Jones JDG

A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell Volume 161, Issue 5, p1089–1100

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Mithoe SC, Menke FL (2015)

Phosphopeptide Immuno-Affinity Enrichment to Enhance Detection of Tyrosine Phosphorylation in Plants. Methods in Molecular Biology Volume 1306, 2015, pp 135-146

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Macho AP, Schwessinger B, Ntoukakis V, Brutus A, Segonzac C, Roy S, Kadota Y, Oh MH, Sklenar J, Derbyshire P, Lozano-Duran R, Gro Malinovsky F, Monaghan J, Menke FL, Huber SC, He SY and Zipfel C (2014)

A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science, 343 (6178): 1509-1512.

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Kadota Y, Sklenar J, Derbyshire P, Stransfeld L, Asai S, Ntoukakis V, Jones JDG, Shirasu K, Menke FL, Jones A and Zipfel C (2014)

Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Molecular Cell, 54 (1): 43-55.

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Mithoe SC, Boersema PJ, Berke L, Snel B, Heck AJ and Menke FL (2012)

Targeted quantitative phosphoproteomics approach for the detection of phospho-tyrosine signaling in plants. J Proteome Res 11, 438-448.

Mithoe SC and Menke FLH (2011)

Phosphoproteomics perspective on plant signal transduction and tyrosine phosphorylation. Phytochemistry 72, 997-1006.

Benschop JJ, Mohammed S, O'Flaherty M, Heck AJR, Slijper M and Menke FLH (2007)

Quantitative phosphoproteomics of early elicitor signaling in arabidopsis. Mol Cell Proteomics 6, 1198-1214

Job History

2013 – present Head of Proteomics, The Sainsbury Laboratory, Norwich, UK.

2006 – 2013 Assistant Professor in Molecular Genetics, Utrecht University, Netherlands.

2003 – 2006 Senior Researchers, Utrecht University, Netherlands.

2002 – 2003 HFSP Postdoctoral Fellow, Utrecht University, Netherlands.

1999 – 2002 HFSP Postdoctoral Fellow, Boyce Thompson Institute, Cornell University, New York, USA.

1994 – 1999 PhD Student, Institute for Plant Molecular Science, Leiden University, Netherlands.