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Dr Joe Win

Postdoctoral Scientist
Sophien Kamoun Group

I am fascinated by nature and evolution of Life (aren’t we all?). To me, nothing is more compelling than observing the arm race between plant pathogens and their hosts that are co-evolving in a vicious cycle of attack and defence: “Gene-for-Gene”, “Boom-and-Bust”, “Guard-Guardee-Decoy Hypothesis”, “Zig-Zag-Zig”,  and “All That Jazz”. Specifically, I am interrogating a notorious plant pathogen called Phytophthora infestans, an oomycete aka the Irish potato famine pathogen, that causes blight disease on potatoes and tomatoes.

We know that this pathogen suppresses plant immunity and manipulates plant physiological processes through the activity of so called effectors that are translocated inside the plant cells, and thought to interact with the plant proteins to do their deeds.

I am performing in planta co-immunoprecipitation experiments using tagged effectors to fish out the interacting plant proteins which are then identified by mass spectrometry, ultimately aiming to unravel the functions of these effectors.

Dr Joe Win

joe.win@tsl.ac.uk
+44 (0) 1603 450049
Research ID: H-2557-2014

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Publications

Nekrasov V, Wang C, Win J, Lanz C, Weigel D, Kamoun S (2017)

Rapid generation of a transgene-free powdery mildew resistant tomato by genome deletion. Sci Rep. 2017 Mar 28;7(1):482. doi: 10.1038/s41598-017-00578-x.

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Islam MT, Croll D, Gladieux P, Soanes DM, Persoons A, Bhattacharjee P, Hossain MS, Gupta DR, Rahman MM, Mahboob MG, Cook N, Salam MU, Surovy MZ, Sancho VB, Maciel JL, NhaniJúnior A, Castroagudín VL, Reges JT, Ceresini PC, Ravel S, Kellner R, Fournier E, Tharreau D, Lebrun MH, McDonald BA, Stitt T, Swan D, Talbot NJ, Saunders DG, Win J, Kamoun S (2016)

Emergence of wheat blast in Bangladesh was caused by a South American lineage of Magnaporthe oryzae. BMC Biol. 2016 Oct 3;14(1):84.

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Islam T, Croll D, Gladieux P, Soanes S, Persoons A, Bhattacharjee P, Hossain S, Gupta D, Rahman MM, Mahboob MG, Cook N, Salam M, Sancho VB, Maciel JN, Nanib A, Castroagudin V, de Assis Reges JT, Ceresini P, Ravel S, Kellner R, Fournier E, Tharreau D, Lebrun MH, McDonald B, Stitt T, Swan D, Talbot N, Saunders D, Win J, Kamoun S (2016)

Emergence of wheat blast in Bangladesh was caused by a South American lineage of Magnaporthe oryzae. bioRxiv preprint June 19, 2016 doi: http://dx.doi.org/10.1101/059832

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Petre B, Saunders DGO, Sklenar J, Lorrain C, Krasileva KV, Win J, Duplessis S, Kamoun S (2016)

Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies. PLoS ONE, February 10, 2016 doi: 10.1371/journal.pone.0149035

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Dagdas YF, Belhaj K, Maqbool A, Chaparro-Garcia A, Pandey P, Petre B, Tabassum N, Cruz-Mireles N, Hughes RK, Sklenar J, Win J, Menke F, Findlay K, Banfield MJ, Kamoun S, Bozkurt TO

An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor. Elife. 2016 Jan 14;5. pii: e10856. doi: 10.7554/eLife.10856

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Petre B, Saunders DGO, Sklenar J, Lorrain C, Krasileva KV, Win j, Duplessis D, Kamoun S (2015)

In planta expression screens of candidate effector proteins from the wheat yellow rust fungus reveal processing bodies as a pathogen-targeted plant cell compartment. bioRxiv preprint November 19, 2015 doi: http://dx.doi.org/10.1101/032276

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Sharma R, Xia X, Cano LM, Evangelisti E, Kemen E, Judelson H, Oome S, Sambles C, van den Hoogen DJ, Kitner M, Klein J, Meijer HJ, Spring O, Win J, Zipper R, Bode HB, Govers F, Kamoun S, Schornack S, Studholme DJ, Van den Ackerveken G, Thines M (2015)

Genome analyses of the sunflower pathogen Plasmopara halstedii provide insights into effector evolution in downy mildews and Phytophthora. BMC Genomics. 2015 Oct 5;16(1):741. doi: 10.1186/s12864-015-1904-7

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Petre B, Lorrain C, Saunders DG, Win J, Sklenar J, Duplessis S, Kamoun S (2015)

Rust fungal effectors mimic host transit peptides to translocate into chloroplasts. Cell Microbiol. 2015 Oct 1. doi: 10.1111/cmi.12530.

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Oliva RF, Cano L, Raffaele S, Win J, Bozkurt TO, Belhaj K, Oh S, Thines M, Kamoun S (2015)

A recent expansion of the RXLR effector gene Avrblb2 is maintained in global populations of Phytophthora infestans indicating different contributions to virulence. Mol Plant Microbe Interact. 2015 Apr 20.

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Petre B, Saunders DG, Sklenar J, Lorrain C, Win J, Duplessis S, Kamoun S (2015)

Candidate effector proteins of the rust pathogen Melampsora larici-populina target diverse plant cell compartments. Mol Plant Microbe Interact. 2015 Feb 4. [Epub ahead of print]

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Saunders, D., Yoshida, K., Sambles, C., Glover, R., Clavijo, B., Corpas, M., Bunting, D., Dong, S., Clark, M., Swarbreck, D., Ayling, S., Bashton, M., Collin, S., Hosoya, T., Edwards, A., Crossman, L., Etherington, G., Win, J., Cano, L., Studholme, D., Downie, J.A., Caccamo, M., Kamoun, S., and MacLean, D. (2014)

Crowdsourced analysis of ash and ash dieback through the Open Ash Dieback project: A year 1 report on datasets and analyses contributed by a self-organising community. bioRxiv, 5:98.

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Saunders, D.G., Win, J., Kamoun, S., and Raffaele, S. (2014)

Two-dimensional data binning for the analysis of genome architecture in filamentous plant pathogens and other eukaryotes. In “Plant-Pathogen Interactions: Methods and Protocols”, P. Birch, J. Jones, and J. Bos, eds. Methods in Molecular Biology, 1127:29-51.

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Nemri, A., Saunders, D.G., Anderson, C., Upadhyaya, N.M., Win, J., Lawrence, G., Jones, D., Kamoun, S., Ellis, J., and Dodds, P. (2014)

The genome sequence and effector complement of the flax rust pathogen Melampsora lini. Frontiers in Plant Science, 5:98.

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Gro Malinovsky, F., Batoux, M., Schwessinger, B., Youn, J.H., Stransfeld, L., Win, J., Kim, S.K. and Zipfel, C. (2014)

Antagonistic regulation of growth and immunity by the Arabidopsis bHLH transcription factor HBI1. Plant Physiology, in press.

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King, S.R.F., McLellan, H., Boevink, P.C., Armstrong, M.R., Bukharova, B., Sukarta, O., Win, J., Kamoun, S., Birch, P.R.J., and Banfield, M.J. (2014)

Phytophthora infestans RXLR effector PexRD2 interacts with host MAPKKKe to suppress plant immune signaling. Plant Cell, in press.

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Dong, S., Stam, R., Cano, L.M., Song, J., Sklenar, J., Yoshida, K., Bozkurt, T.O., Oliva, R., Liu, Z., Tian, M., Win, J., Banfield, M.J., Jones, A.M.E., van der Hoorn, R.A.L., and Kamoun, S. (2014)

Effector specialization in a lineage of the Irish potato famine pathogen. Science, 343:552-555.

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Lin, K., Limpens, E., Zhang, Z., Ivanov, S., Saunders, D.G.O., Mu, D., Pang, E., Cao, H., Cha, H., Lin, T., Zhou, Q., Shang, Y., Li, Y., Sharma, T., van Velzen, R., de Ruijter, N., Aanen, D.K., Win, J., Kamoun, S., Bisseling, T., Geurts, R., and Huang, S. (2014)

Single nucleus genome sequencing reveals high similarity among nuclei of an endomycorrhizal fungus. PLOS Genetics, 10:e1004078.

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Xia, S., Cheng, Y.T., Huang, S., Win, J., Soards, A., Jinn, T.L., Jones, J., Kamoun, S., Chen, S., Zhang, Y., Li, X. 2013.

Regulation of transcription of NB-LRR-encoding genes SNC1 and RPP4 via H3K4 tri-methylation. Plant Physiology, 162:1694-1705.

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Pais, M., Win, J., Yoshida, K., Etherington, G.J., Cano, L.M., Raffaele, S., Banfield, M.J., Jones, A., Kamoun, S., and Saunders, D.G.O. 2013.

From pathogen genomes to host plant processes: the power of plant parasitic oomycetes. Genome Biology, 14:211

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Win, J., Chaparro-Garcia, A., Belhaj, K., Saunders, D.G.O., Yoshida, K., Dong, S., Schornack, S., Zipfel, C., Robatzek, S., Hogenhout, S.A., and Kamoun, S. 2012.

Effector Biology of Plant-associated Organisms: Concepts and Perspectives. Cold Spring Harbor Symposium on Quantitative Biology, 77:235-247.

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Saunders, D.G.O., Breen, S., Win, J., Schornack, S., Hein, I., Bozkurt, T.O., Champouret, N., Vleeshouwers, V.G.A.A., Birch, P.R.J., Gilroy, E.M., and Kamoun, S. 2012.

Host protein BSL1 associates with Phytophthora infestans RXLR effector AVR2 and the Solanum demissum immune receptor R2 to mediate disease resistance. Plant Cell, 24:3420-3434.

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Lamour, K., Mudge, J., Gobena, D., Hurtado-Gonzales, O.P., Schmutz, J., Kuo, A., Miller, N.A., Rice, B.J., Raffaele, S., Cano, L., Bharti, A.K., Donahoo, R.S., Finley, S.L., Huitema, E., Hulvey, J., Platt, D., Salamov, A., Savidor, A., Sharma, R., Stam, R., Storey, D., Thines, M., Win, J., Haas, B., Dinwiddie, D., Jenkins, J., Knight, J., Affourtit, J., Han, C.S., Chertkov, O., Lindquist, E.A., Detter, C., Grigoriev, I.V., Kamoun, S., Kingsmore, S.F. 2012.

Genome sequencing and mapping reveal loss of heterozygosity as a mechanism for rapid adaptation in the vegetable pathogen Phytophthora capsici. Molecular Plant-Microbe Interactions, 25:1350-1360.

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Saitoh, H., Fujisawa, S., Mitsuoka, C., Ito, A., Hirabuchi, A., Ikeda, K., Irieda, H., Yoshino, K., Yoshida, K., Matsumura, H., Tosa, Y., Win, J., Kamoun, S., Takano, Y., and Terauchi, R. 2012.

Large-scale gene disruption in Magnaporthe oryzae identifies MC69, a secreted protein required for infection by monocot and dicot fungal pathogens. PLoS Pathogens, 8:e1002711.

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Win J, Krasileva KV, Kamoun S, Shirasu K, Staskawicz BJ, Banfield MJ (2012)

Sequence divergent RXLR effectors share a structural fold conserved across plant pathogenic oomycete species. PLoS Pathogens, 8:e1002400.

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Saunders, D.G.O., Win, J., Cano, L.M., Szabo, L.J., Kamoun, S., and Raffaele, S. 2012.

Using hierarchical clustering of secreted protein families to classify and rank candidate effectors of rust fungi. PLoS ONE, 6:e16608.

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Bozkurt, T.O., Schornack, S., Win, J., Shindo, T., Ilyas, M., Oliva, R., Cano, L.M., Jones, A.M.E., Huitema, E., van der Hoorn, R.A.L., and Kamoun, S. 2011.

Phytophthora infestans effector AVRblb2 prevents secretion of a plant immune protease at the haustorial interface. Proceedings of the National Academy of Sciences USA, 108:20832-20837.

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Boutemy, L.S., King, S.R.F., Win, J., Hughes, R.K., Clarke, T.A., Blumenschein, T.M.A., Kamoun, S., and Banfield, M.J. 2011.

Structures of Phytophthora RXLR effector proteins: a conserved but adaptable fold underpins functional diversity. Journal of Biological Chemistry, 41:35834-35842.

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Win, J., Kamoun, S., and Jones, A.M. 2011.

Purification of effector-target protein complexes via transient expression in Nicotiana benthamiana. In “Plant Immunity: Methods and Protocols”, J.M. McDowell, ed. Methods in Molecular Biology, 712:181-194

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Tian, M., Win, J., Savory, E., Burkhardt, A., Held, M., Brandizzi, F., Day, B. 2011.

454 genome sequencing of Pseudoperonospora cubensis reveals effector proteins with a putative QXLR translocation motif. Molecular Plant-Microbe Interactions, 24:543-553.

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Baxter, L., Tripathy, S., Ishaque, N., Boot, N., Cabral, A., Kemen, E., Thines, M., Ah-Fong, A., Anderson, R., Badejoko, W., Bittner-Eddy, P., Boore, J.L., Chibucos, M.C., Coates, M., Dehal, P., Delehaunty, K., Dong, S., Downton, P., Dumas, B., Fabro, G., Fronick, C., Fuerstenberg, S.I., Fulton, L., Gaulin, E., Govers, F., Hughes, L., Humphray, S., Jiang, R.H.Y., Judelson, H., Kamoun, S., Kyung, K., Meijer, H., Minx, P., Morris, P., Nelson, J., Phuntumart, V., Qutob, D., Rehmany, A., Rougon-Cardoso, A., Ryden, P., Torto-Alalibo, T., Studholme, D., Wang, Y., Win, J., Wood, J., Clifton, S.W., Rogers, J., Van den Ackerveken, G., Jones, J.D.G., McDowell, J.M., Beynon, J., and Tyler, B.M. 2010.

Signatures of adaptation to obligate biotrophy in the Hyaloperonospora arabidopsidis genome. Science, 330:1549-1551.

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Raffaele, S., Win, J., Cano, L.M., Kamoun, S. 2010.

Analyses of genome architecture and gene expression reveal novel candidate virulence factors in the secretome of Phytophthora infestans. BMC Genomics, 11:637.

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Bos, J.I.B., Prince, D., Pitino, M., Maffei, M.E., Win, J., and Hogenhout, S.A. 2010.

A functional genomics approach identifies candidate effectors from the aphid species Myzus persicae (green peach aphid). PLoS Genetics, 6:e1001216.

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Kaschani, F., Shabab, M., Bozkurt, T., Shindo, T., Schornack, S., Gu, C., Ilyas, M., Win, J., Kamoun, S., van der Hoorn, R.A. 2010.

An effector-targeted protease contributes to defense against Phytophthora infestans and is under diversifying selection in natural hosts. Plant Physiology, 154:1794-1804.

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Levesque, C.A., Brouwer, H., Cano, L., Hamilton, J.P., Holt, C., Huitema, E., Raffaele, S., Robideau, G.P., Thines, M., Win, J., Zerillo, M.M., Beakes, G.W., Boore, J.L., Busam, D., Dumas, B., Ferriera, S., Fuerstenberg, S.I., Gachon, C.M., Gaulin, E., Govers, F., Grenville-Briggs, L., Horner, N., Hostetler, J., Jiang, R.H., Johnson, J., Krajaejun, T., Lin, H., Meijer, H.J., Moore, B., Morris, P., Phuntmart, V., Puiu, D., Shetty, J., Stajich, J.E., Tripathy, S., Wawra, S., van West, P., Whitty, B.R., Coutinho, P.M., Henrissat, B., Martin, F., Thomas, P.D., Tyler, B.M., De Vries, R.P., Kamoun, S., Yandell, M., Tisserat, N., Buell, C.R. 2010.

Genome sequence of the necrotrophic plant pathogen, Pythium ultimum, reveals original pathogenicity mechanisms and effector repertoire. Genome Biology, 11:R73.

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Oliva, R., Win, J., Raffaele, S., Boutemy, L., Bozkurt, T.O., Chaparro-Garcia, A., Segretin, M.E., Stam, R., Schornack, S., Cano, L.M., van Damme, M., Huitema, E., Thines, M., Banfield, M.J., and Kamoun, S. 2010.

Recent developments in effector biology of filamentous plant pathogens. Cellular Microbiology, 12:705-715.

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Schornack, S., Huitema, E., Cano, L.M., Bozkurt, T.O., Oliva, R., van Damme, M., Schwizer, S., Raffaele, S., Chaparro-Garcia, A., Farrer, R., Segretin, M.E., Bos, J., Haas, B.J., Zody, M.C., Nusbaum, C., Win, J., Thines, M., and Kamoun, S. 2009.

Ten things to know about oomycete effectors. Molecular Plant Pathology, 10:795-803.

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Oh, S.-K., Young, C., Lee, M., Oliva, R., Bozkurt, T., Cano, L.M., Win, J., Bos, J.I.B., Liu, H.,-Y., van Damme, M., Morgan, W., Choi, D., van der Vossen, E.A.G., Vleeshouwers, V., and Kamoun, S. 2009.

In planta expression screens of Phytophthora infestans RXLR effectors reveal diverse phenotypes, including activation of the Solanum bulbocastanum disease resistance protein Rpi-blb2. Plant Cell, 21:2028-2947.

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Haas, B.J., Kamoun, S., Zody, M.C., Jiang, R.H.Y., Handsaker, R.E., Cano, L.M., Grabherr, M., Kodira, C.D., Raffaele, S., Torto-Alalibo, T., Bozkurt, T.O., Ah-Fong, A.M.V., Alvarado, L., Anderson, V.L., Armstrong, M.R., Avrova, A., Baxter, L., Beynon, J., Boevink, P.C., Bollmann, S.R., Bos, J.I.B., Bulone, V., Cai, G., Cakir, C., Carrington, J.C., Chawner, M., Conti, L., Costanzo, S., Ewan, R., Fahlgren, N., Fischbach, M.A., Fugelstad, J., Gilroy, E.M., Gnerre, S., Green, P.J., Grenville-Briggs, L.J., Griffith, J., Grunwald, N.J., Horn, K., Horner, N.R., Hu, C.-H., Huitema, E., Jeong, D.-H., Jones, A.M.E., Jones, J.D.G., Jones, R.W., Karlsson, E.K., Kunjeti, S.G., Lamour, K., Liu, Z., Ma, L., MacLean, D., Chibucos, M.C., McDonald, H., McWalters, J., Meijer, H.J.G., Morgan, W., Morris, P.F., Munro, C.A., O'Neill, K., Ospina-Giraldo, M., Pinzon, A., Pritchard, L., Ramsahoye, B., Ren, Q., Restrepo, S., Roy, S., Sadanandom, A., Savidor, A., Schornack, S., Schwartz, D.C., Schumann, U.D., Schwessinger, B., Seyer, L., Sharpe, T., Silvar, C., Song, J., Studholme, D.J., Sykes, S., Thines, M., van de Vondervoort, P.J.I., Phuntumart, V., Wawra, S., Weide, R., Win, J., Young, C., Zhou, S., Fry, W., Meyers, B.C., van West, P., Ristaino, J., Govers, F., Birch, P.R.J., Whisson, S.C., Judelson, H.S., and Nusbaum, C. 2009.

Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature 461:393-398.

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Yoshida, K., Saitoh, H., Fujisawa, S., Kanzaki, H., Matsumura, H., Yoshida, K., Tosa, Y., Chuma, I., Takano, Y., Win, J., Kamoun, S., and Terauchi, R. 2009.

Association genetics reveals three novel avirulence genes from the rice blast fungal pathogen Magnaporthe oryzae. Plant Cell, 21:1573-1591.

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Song, J., Win, J., Tian, M., Schornack, S., Kaschani, F., Ilyas, M., van der Hoorn, R.A.L., and Kamoun, S. 2009.

Two effectors secreted by unrelated eukaryotic plant pathogens target the tomato defense protease Rcr3. Proceedings of the National Academy of Sciences USA, 106:1654-1659.

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Cheung, F., Win, J., Lang, J.M., Hamilton, J., Vuong, H., Leach, J.E., Kamoun, S., Levesque, C.A., Tisserat, N., and Buell, C.R. 2008.

Analysis of the Pythium ultimum transcriptome using Sanger and Pyrosequencing approaches. BMC Genomics, 9:542.

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Damasceno, C.M.B., Bishop, J.G., Ripoll, R.D., Win, J., Kamoun, S., and Rose, J.K.C. 2008.

Structure of the glucanase inhibitor protein (GIP) family from Phytophthora species suggests coevolution with plant endo-beta-1,3-glucanases. Molecular Plant-Microbe Interactions, 21:820-830.

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Morgan, W., Bos, J., Bruce, C., Lee, M., Liu, H.-Y., Oh, S.-K., Song, J., Win, J., Young, C., and Kamoun, S. 2008

Structure and function of RXLR effectors of plant pathogenic oomycetes. In “Genomics of Disease”, J.P. Gustafson, ed. Springer.

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Tian, M.Y., Win, J., Song, J., van der Hoorn, R., van der Knaap, E., and Kamoun, S. (2007).

A Phytophthora infestans cystatin-like protein targets a novel tomato papain-like apoplastic protease. Plant Physiology 143, 364-377.

Win J, Morgan W, Bos J, Krasileva KV, Cano LM, Chaparro-Garcia A, Ammar R, Staskawicz BJ, Kamoun S (2007)

Adaptive evolution has targeted the C-terminal domain of the RXLR effectors of plant pathogenic oomycetes. Plant Cell 19, 2349-2369.

Win, J., Kanneganti, T.D., Torto-Alalibo, T., and Kamoun, S. (2006).

Computational and comparative analyses of 150 full-length cDNA sequences from the oomycete plant pathogen Phytophthora infestans. Fungal Genetics and Biology 43, 20-33.

Win, J., Greenwood, D.R., and Plummer, K.M. (2003).

Characterisation of a protein from Venturia inaequalis that induces necrosis in Malus carrying the Vm resistance gene. Physiological and Molecular Plant Pathology 62, 193-202.

Job History

2007 – present Postdoctoral Scientist, The Sainsbury Laboratory, Norwich, UK.

2003 – 2006 Postdoctoral Fellow, The Ohio State University, USA.

1997 – 1998 Research Technician, School of Medicine, University of Auckland, New Zealand.