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Dr Dan MacLean

Head of Bioinformatics
Bioinformatics

My group is made of researchers and technologists working at the boundary of genomic biology and computer science.

Our exciting projects range from genomics through bioinformatics and citizen science to evolutionary modeling. We bring together biology, computational science and bioinformatics to make the most of big data and help answer big questions.

We provide the computational and software infrastructure to enable bioinformatics work at the highest level for Sainsbury Laboratory researchers at any level of experience.

Working with the Norwich Biosciences Institutes Computing Team and The Genome Analysis Centre, we use high performance compute clusters and high capacity resilient storage to allow us to do science that is not limited by computational resource. 
We provide training and mentoring to biologists to enable them to carry out their own bioinformatics analyses.

Publications

Kawashima CG, Guimarães GA, Nogueira SR, MacLean D, Cook DR, Steuernagel B, Baek J, Bouyioukos C, Melo BD, Tristão G, de Oliveira JC, Rauscher G, Mittal S, Panichelli L, Bacot K, Johnson E, Iyer G, Tabor G, Wulff BB, Ward E, Rairdan GJ, Broglie KE, Wu G, van Esse HP, Jones JD, Brommonschenkel SH (2016)

A pigeonpea gene confers resistance to Asian soybean rust in soybean. Nature Biotechnology (2016) doi:10.1038/nbt.3554

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Page M, MacLean D, Schudoma C (2016)

blastjs: a BLAST+ wrapper for Node.js. BMC Research Notes (2016) 9:130 doi: 10.1186/s13104-016-1938-1

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Corredor-Moreno P, Chalstrey E, Lugo CA, MacLean D (2015)

Identification of Genomic Regions Carrying a Causal Mutation in Unordered Genomes. bioRxiv doi: http://dx.doi.org/10.1101/026856

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Rallapalli G, Fraxinus Players, Saunders DGO, Yoshida K, Edwards A, Lugo CA, Collin S, Clavijo B, Corpas M, Swarbreck D, Clark M, Downie JA, Kamoun S, Team Cooper, MacLean D (2015)

Lessons from Fraxinus, a crowd-sourced citizen science game in genomics. eLife 2015;4:e07460

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Etherington GJ, Ricardo H. Ramirez-Gonzalez RH, MacLean D (2015)

bio-samtools 2: a package for analysis and visualization of sequence and alignment data with SAMtools in Ruby. Bioinformatics (2015) doi: 10.1093/bioinformatics/btv178

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Aleksic J, Alexa A, Attwood TK, Chue Hong N, Dahlö M, Davey R, Dinkel H, Förstner KU, Grigorov I, Hériché JK, Lahti L, MacLean D, Markie ML, Molloy J, Schneider MV, Scott C, Smith-Unna R, Vieira BM (2015)

An open science peer review oath. [v2; ref status: indexed, http://f1000r.es/4wf] F1000Research 2015, 3:271 (doi: 10.12688/f1000research.5686.2)

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Etherington GJ, Monaghan J, Zipfel C, MacLean D (2014)

Mapping mutations in plant genomes with the user-friendly web application CandiSNP. Plant Methods 2014, 10:306. doi:10.1186/s13007-014-0041-7

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Monaghan J, Matschi S, Shorinola O, Rovenich H, Matei A, Segonzac C, Gro Malinovsky F, Rathjen JP, MacLean D, Romeis T, Zipfel C (2014)

The Calcium-Dependent Protein Kinase CPK28 Buffers Plant Immunity and Regulates BIK1 Turnover. Cell Host & Microbe (2014) Volume 16, p605–615. DOI: http://dx.doi.org/10.1016/j.chom.2014.10.007

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Younsi R and MacLean D (2014)

Using 2k + 2 bubble searches to find Single Nucleotide Polymorphisms in k-mer graphs. Bioinformatics 2014 Oct 24. pii: btu706. [Epub ahead of print]

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Leggett RM and MacLean D (2014)

Reference-free SNP detection: dealing with the data deluge. BMC Genomics 2014, 15(Suppl 4):S10 doi:10.1186/1471-2164-15-S4-S10

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Corpas M, Jimenez RC, Bongcam-Rudloff E, Budd A, Brazas MD, Fernandes PL, Gaeta B, van Gelder C, Korpelainen E, Lewitter F, McGrath A, MacLean D, Palagi PM, Rother K, Taylor J, Via A, Watson M, Schneider MV, Attwood TK (2014)

The GOBLET Training Portal: A Global Repository of Bioinformatics Training Materials, Courses and Trainers. Bioinformatics (2014) doi: 10.1093/bioinformatics/btu601

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Ghanasyam Rallapalli, Eric Kemen, Alexandre Robert-Seilaniantz, Cecile Segonzac, Graham Etherington, Kee Sohn, Daniel MacLean, and Jonathan Jones. (2014)

Exprss: an illumina based high-throughput expression-profiling method to reveal transcriptional dynamics. BMC Genomics, 15(1):341

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Saunders, D., Yoshida, K., Sambles, C., Glover, R., Clavijo, B., Corpas, M., Bunting, D., Dong, S., Clark, M., Swarbreck, D., Ayling, S., Bashton, M., Collin, S., Hosoya, T., Edwards, A., Crossman, L., Etherington, G., Win, J., Cano, L., Studholme, D., Downie, J.A., Caccamo, M., Kamoun, S., and MacLean, D. (2014)

Crowdsourced analysis of ash and ash dieback through the Open Ash Dieback project: A year 1 report on datasets and analyses contributed by a self-organising community. bioRxiv, 5:98.

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Dan MacLean. (2014)

Out of the woods. ash dieback and the future of emergent pathogenomics. Molecular Plant Pathology, 15(1):1–4

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Jupe F, Witek K, Verweij W, Sliwka J, Pritchard L, Etherington GJ, Maclean D, Cock PJ, Leggett RM, Bryan GJ, Cardle L, Hein I, Jones JD. (2013)

Resistance gene enrichment sequencing (renseq) enables reannotation of the nb-lrr gene family from sequenced plant genomes and rapid mapping of resistance loci in segregating populations. The Plant Journal, 76(3):530–544

Kosugi, S., Natsume, S., Yoshida, K., MacLean, D., Cano, L., Kamoun, S., and Terauchi, R. 2013.

Coval: Improving alignment quality and variant calling accuracy for next-generation sequencing data. PLOS ONE, 8:e75402.

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Cano, L.M., Raffaele, S., Haugen, R.H., Saunders, D.G.O., Leonelli, L., MacLean, D., Hogenhout, S.A., and Kamoun, S. 2013.

Major transcriptome reprogramming underlies floral mimicry induced by the rust fungus Puccinia monoica in Boechera stricta. PLOS ONE, 8:e75293.

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Dan MacLean. (2013)

Changing the rules of the game. eLife, 2

Graham J Etherington and Daniel MacLean. (2013)

Svgenes: a library for ren- dering genomic features in scalable vector graphic format. Bioinformatics, 29(15):1890–1892

Cantu, D., Segovia, V., Maclean, D., Bayles, R., Chen, X., Kamoun, S., Dubcovsky, J., Saunders, D.G., and Uauy, C. 2013.

Genome analyses of the wheat yellow (stripe) rust pathogen Puccinia striiformis f. sp. tritici reveal polymorphic and haustorial expressed secreted proteins as candidate effectors. BMC Genomics, 14:270.

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MacLean, D., Yoshida, K., Edwards, A., Crossman, L., Clavijo, B., Clark, M., Swarbreck, D., Bashton, M., Chapman, P., Gijzen, M., Caccamo, M., Downie, A., Kamoun, S., and Saunders, D. 2013.

Crowdsourcing genomic analyses of ash and ash dieback — power to the people. GigaScience, 2:2.

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Richard M Leggett, Ricardo H Ramirez-Gonzalez, Walter Verweij, Cintia G Kawashima, Zamin Iqbal, Jonathan DG Jones, Mario Caccamo, and Daniel MacLean. (2013)

Identifying and classifying trait linked polymorphisms in non-reference species by walking coloured de bruijn graphs. PloS ONE, 8(3):e60058

Rhys A Farrer, Daniel A Henk, Dan MacLean, David J Studholme, and Matthew C Fisher. (2013)

Using false discovery rates to benchmark snp-callers in next-generation sequencing projects. Scientific Reports, 3

Beck, M., Zhou, J., Faulkner, C., MacLean, D. and Robatzek, S. (2012)

Spatio-temporal cellular dynamics of the Arabidopsis flagellin receptor reveal activation status-dependent endosomal sorting. Plant Cell. 24: 4205-4219.

Cooke, D.E.L., Cano, L.M., Raffaele, S., Bain, R.A., Cooke, L.R., Etherington, G.J., Deahl, K.L., Farrer, R.A., Gilroy, E.M., Goss, E.M., Grunwald, N.J., Hein, I., MacLean, D., McNicol, J.W., Randall, E., Oliva, R.F., Pel, M.A., Shaw, D.S., Squires, J.N., Taylor, M.C., Vleeshouwers, V.G.A.A., Birch, P.R.J., Lees, A.K., and Kamoun, S. 2012.

Genome Analyses of an Aggressive and Invasive Lineage of the Irish Potato Famine Pathogen. PLoS Pathogens, 8:e1002940.

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GJ Etherington and D MacLean. (2012)

Bioinformatik-analyse von next generation-sequenzierungsdaten. BIOforum-Forschung und Entwicklung, 35(1):34

Ricardo Ramirez-Gonzalez, Raoul JP Bonnal, Mario Caccamo, Daniel MacLean, et al. (2012)

Bio-samtools: Ruby bindings for samtools, a library for accessing bam files containing high-throughput sequence alignments. Source Code for Biology and Medicine, 7(6),

Raoul JP Bonnal, Jan Aerts, George Githinji, Naohisa Goto, Dan MacLean, Chase A Miller, Hiroyuki Mishima, Massimiliano Pagani, Ricardo Ramirez-Gonzalez, Geert Smant, et al. (2012)

Biogem: an effective tool-based approach for scaling up open source software development in bioinformatics. Bioinformatics, 28(7):1035–1037

MacLean, D., and Kamoun, S. 2012.

Big data in small places. Nature Biotechnology, 30:33-34.

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JP Carr, Mathew Lewsey, J Westwood, Heiko Ziebell, Daniel MacLean, A Kleczkowski, M Stevens, and John A Walsh. (2011)

The effects of cucumber mosaic virus 2b protein on methyl jasmonate induced transcriptional re- sponses and plant-aphid interactions in arabidopsis

Ricardo Ramirez-Gonzalez, Mario Caccamo, and Daniel MacLean (2011)

Gee fu: a sequence version and web-services database tool for genomic assembly, genome feature and ngs data. Bioinformatics, 27(19):2754–2755

Eric Kemen, Anastasia Gardiner, Torsten Schultz-Larsen, Ariane C Ke- men, Alexi L Balmuth, Alexandre Robert-Seilaniantz, Kate Bailey, Eric Holub, David J Studholme, Dan MacLean, et al. (2011)

Gene gain and loss during evolution of obligate parasitism in the white rust pathogen of arabidopsis thaliana. PLoS Biology, 9(7):e1001094

Etherington G and MacLean D (2011)

Next-Generation techniques for analysing Next-Generation sequence data (2011). GIT Laboratory Journal, 15, 11-12, pp 10-11

Alexandre Robert-Seilaniantz, Dan MacLean, Yusuke Jikumaru, Lionel Hill, Shinjiro Yamaguchi, Yuji Kamiya, and Jonathan DG Jones. (2011)

The microrna mir393 re-directs secondary metabolite biosynthesis away from camalexin and towards glucosinolates. The Plant Journal, 67(2):218–231

John Walshaw, Graham J Etherington, and Dan MacLean (2011)

Next-generation sequencing approaches to metagenomics. page 63. Horizon Scientific Press

Raffaele, S., Farrer, R.A., Cano, L.M., Studholme, D.J., MacLean, D., Thines, M., Jiang, R.H.Y., Zody, M.C., Kunjeti, S.G., Donofrio, N.M., Meyers, B.C., Nusbaum, C., and Kamoun, S. 2010.

Genome evolution following host jumps in the Irish potato famine pathogen lineage. Science, 330:1540-1543.

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Mathew G Lewsey, Alex M Murphy, Daniel MacLean, Neil Dalchau, Jack H Westwood, Keith Macaulay, Mark H Bennett, Michael Moulin, David E Hanke, Glen Powell, et al. (2010)

Disruption of two defensive signaling pathways by a viral rna silencing suppressor. Molecular Plant-Microbe Interactions, 23(7):835–845.

Salomon, S., Grunewald, D., Stüber, K., Schaaf, S., MacLean, D., Schulze-Lefert, P. and Robatzek, S. (2010)

High throughput confocal imaging of intact live tissue enables quantification of membrane trafficking in Arabidopsis. Plant Physiol. 154: 1096-1104.

Sylviane Comparot-Moss, Oliver Kotting, Michaela Stettler, Christoph Edner, Alexander Graf, Sean E Weise, Sebastian Streb, Wei-Ling Lue, Daniel MacLean, Sebastian Mahlow, et al. (2010)

A putative phosphatase, lsf1, is required for normal starch turnover in arabidopsis leaves. Plant Physiology, 152(2):685–697

MG Lewsey, AM Murphy, D MacLean, N Dalchau, J Westwood, K Macaulay, MH Bennett, M Moulin, DE Hanke, G Powell, et al. (2010)

Disruption of the salicylate and jasmonate signaling pathways by the cucumber mosaic virus 2b rna silencing suppressor. In PHYTOPATHOLOGY, volume 100, pages S70–S70

David J Studholme, Eric Kemen, Daniel MacLean, Sebastian Schornack, Valente Aritua, Richard Thwaites, Murray Grant, Julian Smith, and Jonathan DG Jones. (2010)

Genome-wide sequencing data reveals virulence factors implicated in banana xanthomonas wilt. FEMS Microbiology Letters, 310(2):182–192.

Daniel MacLean, Nataliya Elina, Ericka R Havecker, Susanne B Heim- staedt, David J Studholme, and David C Baulcombe. (2010)

Evidence for large complex networks of plant short silencing rnas. PLoS One, 5(3):e9901

Daniel MacLean, Vincent Moulton, and David J Studholme. (2010)

Finding srna generative locales from high-throughput sequencing data with nibls. BMC Bioinformatics, 11(1):93,

Daniel MacLean and David J Studholme. (2010)

A boolean model of the pseudomonas syringae hrp regulon predicts a tightly regulated system. PloS ONE, 5(2):e9101.

Pawel Durek, Robert Schmidt, Joshua L Heazlewood, Alexandra Jones, Daniel MacLean, Axel Nagel, Birgit Kersten, and Waltraud X Schulze. (2010)

Phosphat: the arabidopsis thaliana phosphorylation site database. an up- date. Nucleic Acids Research, 38(suppl 1):D828–D834

David J Studholme, Selena G Ibanez, Daniel MacLean, Jeffery L Dangl, Jeff H Chang, and John P Rathjen (2009)

A draft genome sequence and functional screen reveals the repertoire of type iii secreted proteins of pseudomonas syringae pathovar tabaci 11528. BMC Genomics, 10(1):395

Haas, B.J., Kamoun, S., Zody, M.C., Jiang, R.H.Y., Handsaker, R.E., Cano, L.M., Grabherr, M., Kodira, C.D., Raffaele, S., Torto-Alalibo, T., Bozkurt, T.O., Ah-Fong, A.M.V., Alvarado, L., Anderson, V.L., Armstrong, M.R., Avrova, A., Baxter, L., Beynon, J., Boevink, P.C., Bollmann, S.R., Bos, J.I.B., Bulone, V., Cai, G., Cakir, C., Carrington, J.C., Chawner, M., Conti, L., Costanzo, S., Ewan, R., Fahlgren, N., Fischbach, M.A., Fugelstad, J., Gilroy, E.M., Gnerre, S., Green, P.J., Grenville-Briggs, L.J., Griffith, J., Grunwald, N.J., Horn, K., Horner, N.R., Hu, C.-H., Huitema, E., Jeong, D.-H., Jones, A.M.E., Jones, J.D.G., Jones, R.W., Karlsson, E.K., Kunjeti, S.G., Lamour, K., Liu, Z., Ma, L., MacLean, D., Chibucos, M.C., McDonald, H., McWalters, J., Meijer, H.J.G., Morgan, W., Morris, P.F., Munro, C.A., O'Neill, K., Ospina-Giraldo, M., Pinzon, A., Pritchard, L., Ramsahoye, B., Ren, Q., Restrepo, S., Roy, S., Sadanandom, A., Savidor, A., Schornack, S., Schwartz, D.C., Schumann, U.D., Schwessinger, B., Seyer, L., Sharpe, T., Silvar, C., Song, J., Studholme, D.J., Sykes, S., Thines, M., van de Vondervoort, P.J.I., Phuntumart, V., Wawra, S., Weide, R., Win, J., Young, C., Zhou, S., Fry, W., Meyers, B.C., van West, P., Ristaino, J., Govers, F., Birch, P.R.J., Whisson, S.C., Judelson, H.S., and Nusbaum, C. 2009.

Genome sequence and analysis of the Irish potato famine pathogen Phytophthora infestans. Nature 461:393-398.

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Vladimir T Valkov, Nunzia Scotti, Sabine Kahlau, Daniel MacLean, Ste- fania Grillo, John C Gray, Ralph Bock, and Teodoro Cardi. (2009)

Genome-wide analysis of plastid gene expression in potato leaf chloroplasts and tuber amyloplasts: transcriptional and posttranscriptional control. Plant Physi- ology, 150(4):2030–2044.

Alexandra ME Jones, Daniel MacLean, David J Studholme, Antonio Serna-Sanz, Erik Andreasson, John P Rathjen, and Scott C Peck. (2009)

Phosphoproteomic analysis of nuclei-enriched fractions from arabidopsis thaliana. Journal of Proteomics, 72(3):439–451

Daniel MacLean, Jonathan DG Jones, and David J Studholme. (2009)

Application of ’next-generation’ sequencing technologies to microbial genetics. Nature Reviews Microbiology, 7(4):287–296

Dan MacLean and David J Studholme (2009)

Bioinformatics aspects of high-throughput sequencing technology. In Plant Pathogenic Bacteria: Genomics and Molecular Biology, page 311. Horizon Scientific Press

Simon Moxon, Frank Schwach, Tamas Dalmay, Dan MacLean, David J Studholme, and Vincent Moulton (2008)

A toolkit for analysing large-scale plant small rna datasets. Bioinformatics, 24(19):2252–2253,

Amanda J Cottage, Ellie K Mott, Jun-Hui Wang, James A Sullivan, Dan MacLean, Linh Tran, Mun-Kit Choy, Christine Newell, Tony A Kavanagh, Sue Aspinall, et al. (2008)

Gun1 (genomes uncoupled1) encodes a pentatricopep- tide repeat (ppr) protein involved in plastid protein synthesis-responsive retrograde signaling to the nucleus. In Photosynthesis. Energy from the Sun, pages 1201–1205. Springer Netherlands.

Daniel MacLean, Michael A Burrell, David J Studholme, and Alexandra ME Jones. (2008)

Phoscalc: a tool for evaluating the sites of peptide phos- phorylation from mass spectrometer data. BMC Research Notes, 1(1):30, 2008.

Daniel MacLean, Cheryl A Jerome, Anthony PC Brown, and John C Gray. (2008)

Co-regulation of nuclear genes encoding plastid ribosomal proteins by light and plastid signals during seedling development in tobacco and arabidopsis. Plant Molecular Biology, 66(5):475–490

Harrold A van den Burg, Dimitrios I Tsitsigiannis, Owen Rowland, Jane Lo, Ghanasyam Rallapalli, Daniel MacLean, Frank LW Takken, and Jonathan DG Jones. (2008)

The f-box protein acre189/acif1 regulates cell death and defense responses activated during pathogen recognition in tobacco and tomato. The Plant Cell Online, 20(3):697–719

Job History

2013 – present Honorary Senior Lecturer, School of Computational Sciences, University of East Anglia, UK.

2010 – present Head of Bioinformatics, The Sainsbury Laboratory, Norwich, UK.

2009 – present Head of Training in Bioinformatics, The Sainsbury Laboratory, Norwich, UK.

2006 – 2010 Bioinformatician, The Sainsbury Laboratory, Norwich, UK.

2005 – 2006 Postdoctoral Researcher, Carnegie Institution, Stanford University, USA.