The Application of RenSeq for Pan-NLRome Analysis

Published: 06.02.26
Authors: Wu Y, Jones JDG, Lin X (2026)
Reference: Methods Mol Biol. 2026;3012:113-127. doi: 10.1007/978-1-0716-5138-4_8

Resistance gene enrichment sequencing (RenSeq) was developed in 2013. It has accelerated the cloning of plant NLR genes and has contributed to resistance breeding for multiple crop plants, such as potato, wheat, and rice. By combining with other strategies, many applications were developed, such as cDNA-RenSeq, dRenSeq, SMRT-RenSeq, RLP/KSeq, AgRenSeq, and MutRenSeq. These methods have been widely applied in different crops. In this protocol, we present a step-by-step guide for applying RenSeq in gene cloning and Pan-NLRome analysis. The protocol covers bait design, library preparation, target enrichment, and downstream bioinformatic analysis. This methodology can make RenSeq more accessible to researchers working with different crops and enhance our understanding of plant resistance genes in the age of pan-genome.

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