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Dr Vladimir Nekrasov

Postdoctoral Scientist
Sophien Kamoun Group

My primary research interest is in genome engineering of plants. 
Genome engineering is a rapidly developing field that encompasses development of strategies and techniques for achieving targeted modification of genomes of various organisms. For example, genome engineering can be used to introduce a specific type of mutation into a genomic locus of interest.

Other applications include adding a fluorescent or affinity tag to a protein expressed at its native location in a genome. Molecular tools used for engineering genomes are site specific nucleases (SSNs), which can be programmed to recognise and cut DNA at a specific genomic site. Most commonly used SSNs are zinc finger nucleases (ZFNs), TAL effector nucleases (TALENs) and Clustered Regularly Interspaced Short Palindromic Repeats (CRISPR)/CRISPR Associated (Cas) RNA-guided nucleases.

Publications

Chaparro-Garcia A, Kamoun S, Nekrasov V (2015)

Boosting plant immunity with CRISPR/Cas. Genome Biol. 2015 Nov 19;16(1):254.

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Schwessinger B, Bahar O, Thomas N, Holton N, Nekrasov V, Ruan D, Canlas PE, Daudi A, Petzold CJ, Singan VR, Kuo R, Chovatia M, Daum C, Heazlewood JL, Zipfel C, Ronald PC (2015)

Transgenic Expression of the Dicotyledonous Pattern Recognition Receptor EFR in Rice Leads to Ligand-Dependent Activation of Defense Responses. PLOS Pathogens: DOI: 10.1371/journal.ppat.1004809

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Holton N, Nekrasov V, Ronald PC, Zipfel C (2015)

The Phylogenetically-Related Pattern Recognition Receptors EFR and XA21 Recruit Similar Immune Signaling Components in Monocots and Dicots. PLoS Pathog. 2015 Jan 21;11(1):e1004602. doi: 10.1371/journal.ppat.1004602.

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Belhaj K, Chaparro-Garcia A, Kamoun S, Patron NJ, Nekrasov V (2015)

Editing plant genomes with CRISPR/Cas9. Current Opinion in Biotechnology Volume 32, April 2015, Pages 76–84

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Brooks C, Nekrasov V, Lippman Z and Van Eck J (2014)

Efficient gene editing in tomato in the first generation using the CRISPR/Cas9 system. Plant Physiol. (2014) Sep 15. pii: pp.114.247577. [Epub ahead of print]

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Belhaj, K., Chaparro-Garcia, A., Kamoun, S. and Nekrasov, V. (2013)

Plant genome editing made easy: targeted mutagenesis in model and crop plants using the CRISPR/Cas system. Plant Methods, 9:39.

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Nekrasov, V., Staskawicz, B., Weigel, D., Jones, J.D.G., and Kamoun, S. (2013)

Targeted mutagenesis in the model plant Nicotiana benthamiana using Cas9 RNA-guided endonuclease. Nature Biotechnology, 31:691-693.

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Batoux M, Lacombe S, Li J, Morehouse K, Nekrasov V, Nicaise V, Niebergall R, Rougon A, Roux M, Schwessinger B, Westedt I, and Zipfel C (2010)

Deciphering PAMP-triggered immunity in Arabidopsis. In: Antoun H, Avis T, Brisson L, Prevost D and Trepanier M (ed). International Society for Molecular Plant-Microbe Interactions, St. Paul, Minnesota. Biology of Plant-Microbe Interactions, 7.

Nekrasov V, Li J, Batoux M, Roux M, Lacombe S, Rougon A, Chu ZH, Bittel P, Kiss-Papp M, Chinchilla D, van Esse HP, Jorda L, Schwessinger B, Nicaise V, Thomma BPHJ, Molina A, Jones JDG and Zipfel C (2009)

Control of the pattern recognition receptor EFR by an ER protein complex in plant immunity. EMBO Journal 28: 3428-3438.

Li J, Chu ZH, Batoux M, Nekrasov V, Roux M, Chinchilla D, Zipfel C and Jones JDG (2009)

A dedicated subset of ER quality control components required for plant innate immunity. Proc. Natl. Acad. Sci. USA 106: 15973-15978.

Vladimir Nekrasov, Andrea A. Ludwig, Jonathan D.G. Jones (2006

CITRX thioredoxin is a putative adaptor protein connecting Cf-9 and the ACIK1 protein kinase during the Cf-9/Avr9- induced defence response. FEBS Letters 580(17):4236-41

O Rowland, AA Ludwig, CJ Merrick, F Baillieul, FE Tracy, WE Durrant et al. (2004)

Functional analysis of Avr9/Cf-9 rapidly elicited genes identifies a protein kinase, ACIK1, that is essential for full Cf-9–dependent disease resistance in tomato. The Plant Cell Online 17 (1), 295-310