Woolfenden HC, Bourdais G, Kopischke M, Miedes E, Molina A, Robatzek S, Morris RJ (2017)
A computational approach for inferring the cell wall properties that govern guard cell dynamics. Plant Journal 92, 5-18.Read more
A paradigm for more than a decade, it has been known that host membranes respond to pathogen perception. It is now clear that the secretory and endocytic trafficking network are engaged by the plant’s immune system to actively defend against potential pathogens. Reciprocally, invasive pathogens have evolved means to utilize these trafficking pathways for the suppression of plant defences and to promote microbial proliferation.
I am particularly interested to understand how host membranes and their cargoes such as the flagellin receptor FLS2 and other pattern recognition receptors (PRRs) are transported through the cell: how this is regulated, how this contributes to PRR abundance control and thus how it regulates defence activation, for example at the level of pathogen-regulated stomatal movements.
“If we are to understand plant-microbe interactions, we need to investigate the associated sub-cellular adaptations at the molecular level.”
To drive our research, we develop methods for quantitative, high-throughput live cell imaging and combine them with genetic and biochemical approaches.
Research Gate: Silke_Robatzek
A computational approach for inferring the cell wall properties that govern guard cell dynamics. Plant Journal 92, 5-18.Read more
Disruption of the plant-specific CFS1 gene impairs autophagosome turnover and triggers EDS1-dependent cell death. Sci Rep. 2017 Aug 17;7(1):8677. doi: 10.1038/s41598-017-08577-8.
An automated quantitative image analysis tool for the identification of microtubule patterns in plants. Traffic. 2017 Jul 26. doi: 10.1111/tra.12505.Read more
A computational approach for inferring the cell wall properties that govern guard cell dynamics. Plant J. 2017 Jul 25. doi: 10.1111/tpj.13640.Read more
Editorial overview: Biotic interactions: Inferring global implications for the molecular interface between plants and their biotic interactions across scales. Curr Opin Plant Biol. 2017 Jul 6. pii: S1369-5266(17)30104-8. doi: 10.1016/j.pbi.2017.06.001.Read more
Detection and Analyses of Endocytosis of Plant Receptor Kinases. Methods Mol Biol. 2017;1621:177-189. doi: 10.1007/978-1-4939-7063-6_17.Read more
Arabidopsis glycosylphosphatidylinositol-anchored protein LLG1 associates with and modulates FLS2 to regulate innate immunity. Proc Natl Acad Sci U S A. 2017 May 15. pii: 201614468. doi: 10.1073/pnas.1614468114.Read more
Plant immune and growth receptors share common signalling components but localise to distinct plasma membrane nanodomains. eLife 2017;10.7554/eLife.25114Read more
Clathrin-dependent endocytosis is required for immunity mediated by pattern recognition receptor kinases. Proc Natl Acad Sci U S A. 2016 Sep 20. pii: 201606004.Read more
The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1. PLoS Pathog. 12(8):e1005811. doi: 10.1371/journal.ppat.1005811.Read more
NB-LRR signaling induces translational repression of viral transcripts and the formation of RNA processing bodies through mechanisms differing from those activated by UV stress and RNAi. J. Exp. Bot. (2016) doi: 10.1093/jxb/erw042Read more
Cell re-entry assays do not support models of pathogen-independent translocation of AvrM and AVR3a effectors into plant cells. bioRxiv preprint January 29, 2016 doi: http://dx.doi.org/10.1101/038232Read more
Biochemical characterization of the tomato phosphatidylinositol-specific phospholipase C (PI-PLC) family and its role in plant immunity. Biochimica et Biophysica Acta (BBA) – Molecular and Cell Biology of Lipids doi:10.1016/j.bbalip.2016.01.017Read more
Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO reports (2016). DOI 10.15252/embr.201540806Read more
Avr4 promotes Cf-4 receptor-like protein association with the BAK1/SERK3 receptor-like kinase to initiate receptor endocytosis and plant immunity. New Phytol. 2016 Jan 14. doi: 10.1111/nph.13802.Read more
Tomato I2 immune receptor can be engineered to confer partial resistance to the oomycete Phytophthora infestans in addition to the fungus Fusarium oxysporum. Mol Plant Microbe Interact. 2015 Sep 14.Read more
Should I stay or should I go? Traffic control for plant pattern recognition receptors. Curr Opin Plant Biol: 28: 23-29. doi: 10.1016/j.pbi.2015.08.007.Read more
A Moving View: Subcellular Trafficking Processes in Pattern Recognition Receptor-Triggered Plant Immunity. Annu Rev Phytopathol. 201; 53:379-402. doi: 10.1146/annurev-phyto-080614-120347.Read more
Phosphorylation of the Plant Immune Regulator RPM1-INTERACTING PROTEIN4 Enhances Plant Plasma Membrane H+-ATPase Activity and Inhibits Flagellin-Triggered Immune Responses in Arabidopsis. Plant Cell. 2015 Jul 21. pii: tpc.114.132308.Read more
Large-Scale Phenomics Identifies Primary and Fine-Tuning Roles for CRKs in Responses Related to Oxidative Stress. PLoS Genet. 11(7):e1005373. doi: 10.1371/journal.pgen.1005373.Read more
Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist 14 Jul 2015 doi: 10.1111/nph.13532Read more
The Cf-4 receptor-like protein associates with the BAK1 receptor-like kinase to initiate receptor endocytosis and plant immunity. bioRxiv preprint May 19, 2015; doi: http://dx.doi.org/10.1101/019471Read more
SOBIR1 requires the GxxxG dimerization motif in its transmembrane domain to form constitutive complexes with receptor-like proteins. Mol Plant Pathol. 2015 Apr 16. doi: 10.1111/mpp.12266.Read more
Identification of regulatory and cargo proteins of endosomal and secretory pathways in Arabidopsis thaliana by proteomic dissection. Mol Cell Proteomics. 2015 Apr 21.Read more
Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato. Nature Plants 1, Article number: 15034 (2015) doi:10.1038/nplants.2015.34Read more
14-3-3 proteins in plant-pathogen interactions. Mol Plant Microbe Interact. 2015 Jan 13. [Epub ahead of print]Read more
The Plasmodesmal Protein PDLP1 Localises to Haustoria-Associated Membranes during Downy Mildew Infection and Regulates Callose Deposition. PLoS Pathog. 2014 Nov 13;10(11):e1004496. doi: 10.1371/journal.ppat.1004496.Read more
Expression patterns of FLAGELLIN SENSING 2 map to bacterial entry sites in plant shoots and roots. J. Exp. Bot. (2014) doi: 10.1093/jxb/eru366Read more
Gate control: guard cell regulation by pathogen stress. New Phytol. Volume 203, Issue 4, pages 1049–1063.Read more
Endocytosis: At the crossroads of pattern recognition immune receptors and pathogen effectors. Applied Plant Cell Biol: Cellular Tools and Approaches for Plant Biotechnology. Springer. In press. (book chapter)
High-throughput imaging of plant immune responses. Methods Mol Biol: Plant Pathogen Interact. Springer. 127:67-80.Read more
The bacterial effector HopM1 suppresses stomatal immunity to promote virulence. New Phytologist. 202: 259-269.Read more
Illuminating traffic control for cell division planes. eLife 3:e02747 (insight article).Read more
Sorting by the ESCRT machinery is required for FLS2 endosomal trafficking and plant immunity. PLoS Gen. 9: e1004035.
The family of AtPeps and their precursors in Arabidopsis: Differential expression and localization but similar induction of pattern-triggered immune responses. J Exp. Bot. 64: 5309-5321.
Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium. Mol Plant Pathol. 15: 109-117.
Guarding the green: Pathways to stomatal immunity. Mol. Plant Microbe Interact. 26: 626-632. (review)
The receptor-like kinase SOBIR1/EVR interacts with receptor-like proteins in plant immunity against fungal infection. Proc. Natl. Acad. Sci. USA. 110: 10010-10015.
LYM2-dependent chitin perception limits molecular flux via plasmodesmata. Proc. Natl. Acad. Sci. USA. 110: 9166-9170.
Salicylic acid interferes with clathrin-mediated, Brefeldin A-sensitive endocytic protein trafficking. Proc. Natl. Acad. Sci. USA. 110:7946-7951.
A developmental framework for complex plasmodesmata formation revealed by large-scale imaging of the Arabidopsis leaf epidermis. Plant Cell. 25: 57-70.
CalloseMeasurer: A Novel Software Solution to Measure Callose Deposition and Recognise Spreading Callose Patterns. Plant Methods. 2012. 8: 49.
Effector Biology of Plant-associated Organisms: Concepts and Perspectives. Cold Spring Harbor Symposium on Quantitative Biology, 77:235-247.Read more
Spatio-temporal cellular dynamics of the Arabidopsis flagellin receptor reveal activation status-dependent endosomal sorting. Plant Cell. 24: 4205-4219.
Mapping FLS2 function to structure: LRRs, kinase and its working bits. Protoplasma. 250: 671-681. (review)
Plants and pathogens: putting infection strategies and defence mechanisms on the map. Curr Opin Plant Biol. 77: 235-247. (review)
The INs and OUTs of pattern recognition receptors at the cell surface. Curr Opin Plant Biol. 15: 367-374. (review)
The shoot apical meristem regulatory peptide CLV3 does not activate innate immunity. Plant Cell, 24(8): 3186-3192.
Crosstalk between PAMP-triggered immunity and photosynthesis. Mol. Plant Microbe Interact. 25: 1083-1092.
ER-Quality Control Chaperones Facilitate the Biogenesis of Cf Receptor-Like Proteins Involved in Pathogen Resistance of Tomato. Plant Physiol. 159: 1819-1833.
Plasma membrane calcium ATPases are important components of receptor-mediated signalling in plant immune responses and development. Plant Physiol. 159: 798-809.
Patterns of plant subcellular responses to successful oomycete infections reveal differences in host cell reprogramming and endocytic trafficking. Cellular Microbiology, 14:682-697Read more
Flagellin perception varies quantitatively in Arabidopsis thaliana and its relatives. Mol. Biol. Evol. 29: 1655-1667.
Receptor trafficking in plants. Signaling and Communication in Plants: Receptor-like Kinases in Plants. Springer. 13: 253-274.
The broad spectrum of plant associations with other organisms. Curr. Opin. Plant Biol. 14: 347-350.
PAMP-induced changes in plasma membrane compartmentalization reveal novel components of plant immunity. J. Biol. Chem. 285: 39140-39149.
High throughput confocal imaging of intact live tissue enables quantification of membrane trafficking in Arabidopsis. Plant Physiol. 154: 1096-1104.
PAMP-triggered immunity: veni, vidi, …? Plant Physiol. 154: 551-554. (review)
Ethylene signalling regulates accumulation of the FLS2 receptor and is required for the oxidative burst contributing to plant immunity. Plant Physiol. 154: 391-400.
Lazarus1, a DUF300 protein, contributes to programmed cell death associated with Arabidopsis acd11 and the hypersensitive response. PLoS One, 5(9). pii: e12586.
Turning the table: plants consume microbes as a source of nutrients. PLoS One 5:e11915.
Transcriptional regulation of the CRK/DUF26 group of Receptor-like protein kinases by ozone and plant hormones in Arabidopsis. BMC Plant Biol. 10: 95.
Pattern recognition receptors require N-glycosylation to mediate plant immunity. Journal Biol. Chem. 285: 4629-4636.
Receptor quality control in the endoplasmic reticulum for plant innate immunity. EMBO J. 4;28(21):3439-49
Plant pattern-recognition receptor FLS2 is directed for degradation by the bacterial ubiquitin ligase AvrPtoB. Curr Biol. 2008 Dec 9;18(23):1824-32.
A flagellin-induced complex of the receptor FLS2 and BAK1 initiates plant defence. Nature, 448: 497-500.
Receptor-like kinases and innate immunity in plants. In: Sanchez F., Geiger O. (ed). International Society for Molecular Plant-Microbe Interactions, St. Paul, Minnesota. Biology of Plant-Microbe Interactions, 5: 21-29.
The N-terminus of bacterial elongation factor Tu elicits innate immunity in Arabidopsis plants. Plant Cell, 16 (12): 3496-3507.
The transcriptional innate immune responses to flg22. Interplay and overlap with Avr gene-dependent defense responses and bacterial pathogenesis. Plant Physiology, 135: 1113-1128.
Bacterial disease resistance through flagellin perception in Arabidopsis. Nature, 428: 764-767.
mRNA expression profiling of the Arabidopsis flg22-triggered innate immune response and the Avr9/Cf-9 response in tobacco. In: Tikhonovich I, Lugtenberg B, and Provorov N (ed). International Society for Molecular Plant-Microbe Interactions, St. Paul, Minnesota. Biology of Plant-Microbe Interactions, 4: 237-239.
2009 – present Group Leader, The Sainsbury Laboratory
2009 – present Honorary Professor (Habilitation in Cell Biology 2009), Botanical Institute, University of Basel, Switzerland
2005 – 2009 Group Leader,
2001 – 2005 Postdoctoral Fellow, Friedrich-Miescher Institute for Biomedical Research & Botanical Institute, University of Basel, Switzerland
2000 – 2001 Postdoctoral Fellow, Max-Planck-Institute for Plant Breeding Research, Cologne, Germany
1997 – 2000 PhD Student (PhD in Biochemistry 2000), Max-Planck-Institute for Plant Breeding Research, Cologne, Germany
1991 – 1997 Undergraduate Student (Diploma in Biology 1997), University of Göttingen, Germany