Dr Paul Derbyshire

Proteomics Support Specialist

As part of the Proteomics Group I specialise in targeted proteomics and use Selected Reaction Monitoring (SRM) to quantify post-translational modifications (e.g. phosphorylation, acetylation) of plant proteins following elicitation by pathogens. I am also part of the discovery platform used for the identification of the protein components of samples, including their post-translational modifications.

Other research interests include:

Plant cell walls and growth. Microtubules and secondary wall patterning. Root development. Leaf patterning. Starch metabolism.

Dr Paul Derbyshire
+44 (0) 1603 450225


Abel NB, Buschle CA, Hernandez-Ryes C, Burkart SS, Deroubaix AF, Mergner J, Gronnier J, Jarsch IK, Folgmann J, Braun KH, Bayer E, Germain V, Derbyshire P, Menke FLH, Kemmerling B, Zipfel C, Küster B, Mongrand S, Marín M, Ott T (2021)

A hetero-oligomeric remorin-receptor complex regulates plant development. bioRxiv preprint Jan 29, 2021 doi:

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Grubb LE, Derbyshire P, Dunning KE, Zipfel C, Menke FLH, Monaghan J (2021)

Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings. Plant Physiol. 2021 Jan 28:kiab023. doi: 10.1093/plphys/kiab023.

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Goto Y, Maki N, Sklenar J, Derbyshire P, Menke FLH, Zipfel C, Kadota Y, Shirasu K (2020)

The phagocytosis oxidase/Bem1p (PB1) domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity. bioRxiv preprint Dec 29, 2020 doi:

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Sun X, Lapin D, Feehan JM, Stolze SC, Kramer K, Dongus JA, Rzemieniewski J, Blanvillain-Baufume S, Harzen A, Bautor J, Derbyshire P, Menke FLH, Finkemeier I, Nakagami H, Jones JDG, Parker JE (2020)

Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. bioRxiv preprint Dec 22, 2020 doi:

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Derkacheva M, Yu G, Rufian JS, Jiang S, Derbyshire P, Morcillo R, Stransfeld L, Wei Y, Menke F, Zipfel C, Macho A (2020)

The Arabidopsis E3 ubiquitin ligase PUB4 regulates BIK1 homeostasis and is targeted by a bacterial type-III effector. bioRxiv preprint Oct 26, 2020 doi:

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Grubb L, Derbyshire P, Dunning K, Zipfel C, Menke FLH, Monaghan J (2020)

Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings. bioRxiv preprint Sept 17, 2020 doi:

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Thor K, Jiang S, Michard E, George J, Scherzer S, Huang S, Dindas J, Derbyshire P, Leitão N, DeFalco TA, Köster P, Hunter K, Kimura S, Gronnier J, Stransfeld L, Kadota Y, Bücherl CA, Charpentier M, Wrzaczek M, MacLean D, Oldroyd GED, Menke FLH, M. Roelfsema MRG, Hedrich R, Feijó J, Zipfel C (2020)

The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature. 25 August 2020. DOI: 10.1038/s41586-020-2702-1

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Oses-Ruiz M, Martin-Urdiroz M, Soanes DM, Kershaw MJ, Cruz-Mireles N, Valdovinos-Ponce G, Molinari C, Littlejohn GR, Derbyshire P, Menke F, Valent B, Talbot NJ (2020)

A hierarchical transcriptional network controls appressorium-mediated plant infection by the rice blast fungus Magnaporthe oryzae. bioRxiv preprint Feb 06, 2020 doi:

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Wong JEMM, Nadzieja M, Madsen LH, Bücherl CA, Dam S, Sandal NN, Couto D, Derbyshire P, Uldum-Berentsen M, Schroeder S, Schwämmle V, Nogueira FCS, Asmussen MH, Thirup S, Radutoiu S, Blaise M, Andersen KR, Menke FLH, Zipfel C, Stougaard J (2019)

A Lotus japonicus cytoplasmic kinase connects Nod factor perception by the NFR5 LysM receptor to nodulation. Proc Natl Acad Sci U S A. 2019 Jun 25. pii: 201815425. doi: 10.1073/pnas.1815425116.

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Kadota Y, Liebrand TWH, Goto Y, Sklenar J, Derbyshire P, Menke FLH, Torres MA, Molina A, Zipfel C, Coaker G, Shirasu K (2018)

Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector- and PAMP-triggered immunity in plants. New Phytol. 2018 Oct 9. doi: 10.1111/nph.15523.

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Perraki A, DeFalco TA, Derbyshire P, Avila J, Séré D, Sklenar J, Qi X, Stransfeld L, Schwessinger B, Kadota Y, Macho AP, Jiang S, Couto D, Torii KU, Menke FLH, Zipfel C (2018)

Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature, 3 Sept 2018. doi: 10.1038/s41586-018-0471-x

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Bender KW, Blackburn RK, Monaghan J, Derbyshire P, Menke FL, Zipfel C, Goshe MB, Zielinski RE, Huber SC (2017)

Autophosphorylation-based calcium (Ca2+) sensitivity priming and Ca2+/Calmodulin inhibition of Arabidopsis thaliana Ca2+-dependent protein kinase 28 (CPK28). J Biol Chem. 2017 Jan 30. pii: jbc.M116.763243. doi: 10.1074/jbc.M116.763243.

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Couto D, Niebergall R, Liang X, Bücherl CA, Sklenar J, Macho AP, Ntoukakis V, Derbyshire P, Altenbach D, Maclean D, Robatzek S, Uhrig J, Menke F, Zhou JM, Zipfel C (2016)

The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1. PLoS Pathog. 12(8):e1005811. doi: 10.1371/journal.ppat.1005811.

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Mithoe SC, Ludwig C, Pel MJC, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CMJ, Aebersold R, Menke FLH (2015)

Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO reports (2016). DOI 10.15252/embr.201540806

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Sarris PF, Duxbury Z, Huh SU, Yan Ma, Segonzac C, Sklenar J, Derbyshire P, Cevik V, Rallapalli G, Saucet SB, Wirthmueller L, Menke FLH, Sohn KH, Jones JDG

A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell Volume 161, Issue 5, p1089–1100

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Macho AP, Schwessinger B, Ntoukakis V, Brutus A, Segonzac C, Roy S, Kadota Y, Oh MH, Sklenar J, Derbyshire P, Lozano-Duran R, Gro Malinovsky F, Monaghan J, Menke FL, Huber SC, He SY and Zipfel C (2014)

A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science, 343 (6178): 1509-1512.

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Kadota Y, Sklenar J, Derbyshire P, Stransfeld L, Asai S, Ntoukakis V, Jones JDG, Shirasu K, Menke FL, Jones A and Zipfel C (2014)

Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Molecular Cell, 54 (1): 43-55.

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Derbyshire P., Byrne M. E. (2013)

MORE SPIKELETS1 Is Required for Spikelet Fate in the Inflorescence of Brachypodium. Plant Physiology 161, 1291-302

Moschopoulos A., Derbyshire P., Byrne M. E. (2012)

The Arabidopsis organelle-localized glycyl-tRNA synthetase encoded by EMBRYO DEFECTIVE DEVELOPMENT1 is required for organ patterning Journal of Experimental Botany 63 5233-5243

Barratt D. H., Derbyshire P., Findlay K., Pike M., Wellner N., Lunn J., Feil R., Simpson C., Maule A. J., Smith A. M. (2009)

Normal growth of Arabidopsis requires cytosolic invertase but not sucrose synthase. Proceedings of the National Academy of Sciences of the United States of America 106, 13124-13129

Drea S., Derbyshire P., Koumproglou R., Dolan L., Doonan J. H., Shaw P.(2009)

In situ analysis of gene expression in plants Methods in Molecular Biology 513 229-42

Lee Y., Derbyshire P., Knox J. P., Hvoslef-Eide A. K. (2008)

Sequential cell wall transformations in response to the induction of a pedicel abscission event in Euphorbia pulcherrima (poinsettia). Plant Journal 6, 993-1003

Derbyshire P., Drea S., Shaw P. J., Doonan J. H., Dolan L. (2008)

Proximal-distal patterns of transcription factor gene expression during Arabidopsis root development. Journal of Experimental Botany 59, 235-245

Li Y., Smith C. H., Corke F., Zheng L., Merali Z., Ryden P., Derbyshire P., Waldron K., Bevan M. W. (2007)

Signaling from an altered cell wall to the nucleus mediates sugar-responsive growth and development in Arabidopsis thaliana. Plant Cell 19, 2500-15

Derbyshire P., McCann M. C., Roberts K. (2007)

Restricted cell elongation in Arabidopsis hypocotyls is associated with a reduced average pectin esterification level. BMC Plant Biology 7, article 31

Derbyshire P., Findlay K., McCann M. C., Roberts K. (2007)

Cell elongation in Arabidopsis hypocotyls involves dynamic changes in cell wall thickness. Journal of Experimental Botany 58 2079-2089

Carol R. J., Takeda S., Linstead P., Durrant M. C., Kakesova H., Derbyshire P., Drea S., Zarsky V., Dolan L. (2005)

A RhoGDP dissociation inhibitor spatially regulates growth in root hair cells. Nature 438, 1013-1016

Schindelman G., Morikami A., Jung J., Baskin T. I., Carpita N. C., Derbyshire P., McCann M. C., Benfey P. N. (2001)

COBRA encodes a putative GPI-anchored protein, which is polarly localized and necessary for oriented cell expansion in Arabidopsis. Genes & Development 15, 1115-1127