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Dr Oleg Raitskin

Postdoctoral Scientist
Synthetic Biology

My overall goal within the OpenPlant project, a collaborative research effort between University of Cambridge and the JIC, will be to improve the efficiency of the genome manipulation in plants. The main focus will be on recently emerged CRISPR/Cas9- mediated methods of genome manipulation methods, which are based on the sgRNA molecule, that recognizes target DNA sequence and recruits Cas9 endonuclease to perform cleavage of this DNA sequence. Major lines of my research will include:

– Efficient and precise excision of genomic fragments in vivo with potentially low level of off-target cuts. This will be explored by analysing genomic DNA cut efficiency by different configurations of sgRNA and different transcriptional units of Cas9.

– Possibility of precise manipulation of genomic DNA in vivo, including introducing mutations and tags. This will be explored by attempting to mutate or “tag” endogenous DNA sequences employing homologous or non-homologous recombination mechanisms, facilitated by CRISPR/Cas9 with different templates.

oleg.raitskin@tsl.ac.uk
+44 (0) 1603 450052

Publications

Raitskin O, Patron NJ (2015)

Multi-gene engineering in plants with RNA-guided Cas9 nuclease. Curr Opin Biotechnol. 2015 doi: 10.1016/j.copbio.2015.11.008

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Patron NJ, Orzaez D, Marillonnet S, Warzecha H, Matthewman C, Youles M, Raitskin O, Leveau A, Farré G, Rogers C, Smith A, Hibberd J, Webb AAR, Locke J, Schornack S, Ajioka J, Baulcombe DC, Zipfel C, Kamoun S, Jones JDG, Kuhn H, Robatzek S, Van Esse HP, Sanders D, Oldroyd G, Martin C, Field R, O'Connor S, Fox S, Wulff B, Miller B, Breakspear A, Radhakrishnan G, Delaux PM, Loqué D, Granell A, Tissier A, Shih P, Brutnell TP, Quick WP, Rischer H, Fraser PD, Aharoni A, Raines C, South PF, Ané JM, Hamberger BR, Langdale J, Stougaard J, Bouwmeester H, Udvardi M, Murray JAH, Ntoukakis V, Schäfer P, Denby K, Edwards KJ, Osbourn A, Haseloff J (2015)

Standards for plant synthetic biology: a common syntax for exchange of DNA parts. New Phytologist 14 Jul 2015 doi: 10.1111/nph.13532

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Job History

2011 – 2014 Postdoctoral researcher, John Innes Centre, Norwich, UK

2007 – 2011 Postdoctoral researcher, Hebrew University of Jerusalem, Israel