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Dr Jan Sklenar

Proteomics Support Specialist
Proteomics

I specialize in proteomics and mass spectrometry (MS), my broader background is biochemistry.

The molecular structure of biomolecules can be inferred from the measurement of their accurate masses and collision spectra. In proteomics the most advanced MS instruments are capable high throughput analysis when coupled with a powerful separation technique. I combine affinity enrichment, electrophoresis, and liquid chromatography. My MS knowledge supports hands-on experience with platforms equipped with MALDI and nanoESI ion source: TOF-TOF, qTOF, QQQ, ion trap, and LTQ-Orbitrap.

I have been involved in protein isolation and characterization, including PTM (glycosylation, phosphorylation, and acetylation). I also designed isolation and detection of fungal secondary metabolite from culture filtrates, and separation science with purification of biomolecules is my second area of interest.

Currently I contribute to several proteomics projects in plant pathology lab (TSL) with protein and peptide identification including experimental design, carrying out the measurements, and the data interpretation.

jan.sklenar@tsl.ac.uk
+44 (0) 1603 450225

Publications

Zess EK, Jensen C, Cruz-Mireles N, De la Concepcion JC, Sklenar J, Stephani M, Imre R, Roitinger E, Hughes R, Belhaj K, Mechtler K, Menke FLH, Bozkurt T, Banfield MJ, Kamoun S, Maqbool A, Dagdas YF (2019)

N-terminal β-strand underpins biochemical specialization of an ATG8 isoform. PLoS Biol. 2019 Jul 22;17(7):e3000373. doi: 10.1371/journal.pbio.3000373.

Zess EK, Jensen C, Cruz-Mireles N, De la Concepcion JC, Sklenar J, Imre R, Roitinger E, Hughes R, Belhaj K, Mechtler K, Menke F, Bozkurt T, Banfield M, Kamoun S, Maqbool A, Dagdas Y (2018)

N-terminal β-strand underpins biochemical specialization of an ATG8 isoform. bioRxiv preprint Oct 25, 2018 doi: https://doi.org/10.1101/453563

Kadota Y, Liebrand TWH, Goto Y, Sklenar J, Derbyshire P, Menke FLH, Torres MA, Molina A, Zipfel C, Coaker G, Shirasu K (2018)

Quantitative phosphoproteomic analysis reveals common regulatory mechanisms between effector- and PAMP-triggered immunity in plants. New Phytol. 2018 Oct 9. doi: 10.1111/nph.15523.

Perraki A, DeFalco TA, Derbyshire P, Avila J, Séré D, Sklenar J, Qi X, Stransfeld L, Schwessinger B, Kadota Y, Macho AP, Jiang S, Couto D, Torii KU, Menke FLH, Zipfel C (2018)

Phosphocode-dependent functional dichotomy of a common co-receptor in plant signalling. Nature, 3 Sept 2018. doi: 10.1038/s41586-018-0471-x

Wirthmueller L, Asai S, Rallapalli G, Sklenar J, Fabro G, Kim DS, Lintermann R, Jaspers P, Wrzaczek M, Kangasjärvi J, MacLean D, Menke FLH, Banfield MJ, Jones JDG (2018)

Arabidopsis downy mildew effector HaRxL106 suppresses plant immunity by binding to RADICAL-INDUCED CELL DEATH1. New Phytol. 2018 Oct;220(1):232-248. doi: 10.1111/nph.15277.

Wirthmueller L, Asai S, Rallapalli G, Sklenar J, Fabro G, Kim DS, Lintermann R, Jaspers P, Wrzaczek M, Kangasjärvi L, MacLean D, Menke FLH, Banfield MJ, JDG (2017)

An Oomycete Effector Protein Induces Shade Avoidance In Arabidopsis And Attenuates Salicylate Signaling By Binding To Host Proteins Of The RADICAL-INDUCED CELL DEATH1 Family. bioRxiv preprint May 14, 2017 doi: https://doi.org/10.1101/137844

Couto D, Niebergall R, Liang X, Bücherl CA, Sklenar J, Macho AP, Ntoukakis V, Derbyshire P, Altenbach D, Maclean D, Robatzek S, Uhrig J, Menke F, Zhou JM, Zipfel C (2016)

The Arabidopsis Protein Phosphatase PP2C38 Negatively Regulates the Central Immune Kinase BIK1. PLoS Pathog. 12(8):e1005811. doi: 10.1371/journal.ppat.1005811.

Vit O, Man P, Kadek A, Hausner J, Sklenar J, Harant K, Novak P, Scigelova M, Woffendin G, Petrak J (2016)

Large-scale identification of membrane proteins based on analysis of trypsin-protected transmembrane segments. J Proteomics. 2016 Mar 11. pii: S1874-3919(16)30069-0. doi: 10.1016/j.jprot.2016.03.016.

Saur IM, Kadota Y, Sklenar J, Holton NJ, Smakowska E, Belkhadir Y, Zipfel C, Rathjen JP (2016)

NbCSPR underlies age-dependent immune responses to bacterial cold shock protein in Nicotiana benthamiana. Proc Natl Acad Sci U S A. 2016 Mar 4. pii: 201511847.

Petre B, Saunders DGO, Sklenar J, Lorrain C, Krasileva KV, Win J, Duplessis S, Kamoun S (2016)

Heterologous Expression Screens in Nicotiana benthamiana Identify a Candidate Effector of the Wheat Yellow Rust Pathogen that Associates with Processing Bodies. PLoS ONE, February 10, 2016 doi: 10.1371/journal.pone.0149035

Petre B, Hecker A, Germain H, Tsan P, Sklenar J, Pelletier G, Séguin A, Duplessis S, Rouhier N (2016)

The poplar Rust-Induced Secreted Protein (RISP) inhibits the growth of the leaf rust pathogen Melampsora larici-populina and triggers cell culture alkalinisation. Front. Plant Sci. | doi: 10.3389/fpls.2016.00097

Mithoe SC, Ludwig C, Pel MJC, Cucinotta M, Casartelli A, Mbengue M, Sklenar J, Derbyshire P, Robatzek S, Pieterse CMJ, Aebersold R, Menke FLH (2015)

Attenuation of pattern recognition receptor signaling is mediated by a MAP kinase kinase kinase. EMBO reports (2016). DOI 10.15252/embr.201540806

Dagdas YF, Belhaj K, Maqbool A, Chaparro-Garcia A, Pandey P, Petre B, Tabassum N, Cruz-Mireles N, Hughes RK, Sklenar J, Win J, Menke F, Findlay K, Banfield MJ, Kamoun S, Bozkurt TO

An effector of the Irish potato famine pathogen antagonizes a host autophagy cargo receptor. Elife. 2016 Jan 14;5. pii: e10856. doi: 10.7554/eLife.10856

Petre B, Saunders DGO, Sklenar J, Lorrain C, Krasileva KV, Win j, Duplessis D, Kamoun S (2015)

In planta expression screens of candidate effector proteins from the wheat yellow rust fungus reveal processing bodies as a pathogen-targeted plant cell compartment. bioRxiv preprint November 19, 2015 doi: http://dx.doi.org/10.1101/032276

Gupta YK, Dagdas YF, Martinez-Rochaa A-L, Kershaw MJ, Littlejohn GR, Rydera LS, Sklenar J, Menke F, Talbot NJ (2015)

Septin-Dependent Assembly of the Exocyst Is Essential for Plant Infection by Magnaporthe oryzae. The Plant Cell doi: http://dx.doi.org/10.1105/tpc.15.00552

Petre B, Lorrain C, Saunders DG, Win J, Sklenar J, Duplessis S, Kamoun S (2015)

Rust fungal effectors mimic host transit peptides to translocate into chloroplasts. Cell Microbiol. 2015 Oct 1. doi: 10.1111/cmi.12530.

Chaparro-Garcia A, Schwizer S, Sklenar J, Yoshida K, Petre B, Bos JI, Schornack S, Jones AM, Bozkurt TO, Kamoun S (2015)

Phytophthora infestans RXLR-WY Effector AVR3a Associates with Dynamin-Related Protein 2 Required for Endocytosis of the Plant Pattern Recognition Receptor FLS2. PLoS One;10(9):e0137071. doi: 10.1371/journal.pone.0137071.

Sarris PF, Duxbury Z, Huh SU, Yan Ma, Segonzac C, Sklenar J, Derbyshire P, Cevik V, Rallapalli G, Saucet SB, Wirthmueller L, Menke FLH, Sohn KH, Jones JDG

A plant immune receptor detects pathogen effectors that target WRKY transcription factors. Cell Volume 161, Issue 5, p1089–1100

Heard W, Sklenar J, Tome DF, Robatzek S, Jones AM (2015)

Identification of regulatory and cargo proteins of endosomal and secretory pathways in Arabidopsis thaliana by proteomic dissection. Mol Cell Proteomics. 2015 Apr 21.

Petre B, Saunders DG, Sklenar J, Lorrain C, Win J, Duplessis S, Kamoun S (2015)

Candidate effector proteins of the rust pathogen Melampsora larici-populina target diverse plant cell compartments. Mol Plant Microbe Interact. 2015 Feb 4. [Epub ahead of print]

Caillaud MC, Wirthmueller L, Sklenar J, Findlay K, Piquerez SJ, Jones AM, Robatzek S, Jones JD, Faulkner C (2014)

The Plasmodesmal Protein PDLP1 Localises to Haustoria-Associated Membranes during Downy Mildew Infection and Regulates Callose Deposition. PLoS Pathog. 2014 Nov 13;10(11):e1004496. doi: 10.1371/journal.ppat.1004496.

Wirthmueller L, Roth C, Fabro G, Caillaud MC, Rallapalli G, Asai S, Sklenar J, Jones AM, Wiermer M, Jones JD, Banfield MJ. (2014)

Probing formation of cargo/importin-α transport complexes in plant cells using a pathogen effector. Plant Journal (2014) doi: 10.1111/tpj.12691

Macho AP, Schwessinger B, Ntoukakis V, Brutus A, Segonzac C, Roy S, Kadota Y, Oh MH, Sklenar J, Derbyshire P, Lozano-Duran R, Gro Malinovsky F, Monaghan J, Menke FL, Huber SC, He SY and Zipfel C (2014)

A bacterial tyrosine phosphatase inhibits plant pattern recognition receptor activation. Science, 343 (6178): 1509-1512.

Kadota Y, Sklenar J, Derbyshire P, Stransfeld L, Asai S, Ntoukakis V, Jones JDG, Shirasu K, Menke FL, Jones A and Zipfel C (2014)

Direct regulation of the NADPH oxidase RBOHD by the PRR-associated kinase BIK1 during plant immunity. Molecular Cell, 54 (1): 43-55.

Dong, S., Stam, R., Cano, L.M., Song, J., Sklenar, J., Yoshida, K., Bozkurt, T.O., Oliva, R., Liu, Z., Tian, M., Win, J., Banfield, M.J., Jones, A.M.E., van der Hoorn, R.A.L., and Kamoun, S. (2014)

Effector specialization in a lineage of the Irish potato famine pathogen. Science, 343:552-555.

Liebrand, T.W., Kombrink, A., Zhang, Z., Sklenar, J., Jones, A.M., Robatzek, S., Thomma, B.P. and Joosten, M.H. (2013)

Chaperones of the endoplasmic reticulum are required for Ve1-mediated resistance to Verticillium. Mol Plant Pathol. 15: 109-117.

van Damme, M., Bozkurt, T.O., Cakir, C., Schornack, S., Sklenar, J., Jones, A.E.M., and Kamoun, S. (2012)

The Irish potato famine pathogen Phytophthora infestans translocates the CRN8 Kinase into host plant cells. PLoS Pathogens, 8:e1002875.

Göhre, V., Jones, A.M.E., Sklenář, J., Robatzek, S. and Weber, A.P.M. (2012)

Crosstalk between PAMP-triggered immunity and photosynthesis. Mol. Plant Microbe Interact. 25: 1083-1092.

Liebrand, T.W., Smit, P., Abd-El-Haliem, A., de Jonge, R., Cordewener, J.H., America, A.H., Sklenar, J., Jones, A.M., Robatzek, S., Thomma, B.P., Tameling, W.I. and Joosten, M.H. (2012)

ER-Quality Control Chaperones Facilitate the Biogenesis of Cf Receptor-Like Proteins Involved in Pathogen Resistance of Tomato. Plant Physiol. 159: 1819-1833.

Schwessinger B, Roux M, Kadota Y, Ntoukakis V, Sklenar J, Jones AM and Zipfel C (2011)

Phosphorylation-dependent differential regulation of plant growth, cell death and innate immunity by the regulatory receptor-like kinase BAK1. PLoS Genetics, 7(4): e1002046.

Pavlaskova K, Nedved J, Kuzma M, Zabka M, Sulc M, Sklenar J, Novak P, Benada O, Kofronova O, Hajduch M, Derrick PJ, Lemr K, Jegorov A, Havlicek V (2010)

Characterization of Pseudacyclins A-E, a Suite of Cyclic Peptides Produced by Pseudallescheria boydii J. Nat. Prod. 2010, 73: 1027–1032

Sklenar J, Niku-Paavola ML, Santos S, Man P, Kruus K, Novotny C (2010)

Isolation and characterization of novel pI 4.8 MnP isoenzyme from white-rot fungus Irpex lacteus Enzyme and Microbial Technology 2010, 46: 550–556

Plıhal O, Sklenar J, Hofbauerova K, Novak P, Man P, Pompach P, Kavan D, Ryslava H, Weignerova L, Charvatova-Pisvejcova A, Kren V, Bezouska K (2007)

Large Propeptides of Fungal -N-Acetylhexosaminidases Are Novel Enzyme Regulators That Must Be Intracellularly Processed to Control Activity, Dimerization, and Secretion into the Extracellular Environment† Biochemistry 2007, 46: 2719-2734

Job History

2009 – present Proteomics Support Specialist, The Sainsbury Laboratory, Norwich, UK.

2006 – 2009 Postdoctoral Scientist, Institute of Microbiology, Prague, Czech Republic.

1996 – 2005 Predoctoral Scientist, Charles University, Prague, Czech Republic.