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Dr Hari Karki

Postdoctoral Scientist
Jonathan Jones Group

I am interested in engineering durable disease resistance in crops to feed a growing population.

During my PhD at Louisiana State University, I worked on functional genomics of the rice pathogenic bacteria, Burkholderia glumae and rice breeding for disease resistance. Then, I joined Purdue University as a Post-doctoral researcher and worked on understanding the genetic and biochemical interactions of maize-western corn rootworm beetle.

Here at TSL, I am working on the recently developed Renseq (Resistance gene enrichment sequencing) method to clone functional resistance genes against potato late blight in wild Solanum species.

Publications

Karki HS, Ham JH (2016)

Testing the Effect of UV Radiation on the Survival of Burkholderia glumae. Bio-protocol 6(5): e1755.

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Shrestha BK, Karki HS, Groth DE, Jungkhun N, Ham JH (2016)

Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice. PLoS One. 2016 Jan 14;11(1):e0146764. doi: 10.1371/journal.pone.0146764.

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Karki HS, Ham JH (2014)

The roles of the shikimate pathway genes, aroA and aroB, in virulence, growth and UV tolerance of Burkholderia glumae strain 411gr-6. Mol Plant Pathol 15: 940-7. doi: 10.1111/mpp.12147.

Chen R, Barphagha IK, Karki HS, Ham JH (2012)

Dissection of quorum-sensing genes in Burkholderia glumae reveals non-canonical regulation and the new regulatory gene tofM for toxoflavin production. PLoS One. 2012;7(12):e52150. doi: 10.1371/journal.pone.0052150.

Karki HS, Shrestha BK, Han JW, Groth DE, Barphagha IK, Rush MC, Melanson RA, Kim BS, Ham JH (2012)

Diversities in virulence, antifungal activity, pigmentation and DNA fingerprint among strains of Burkholderia glumae. PLoS One. 2012;7(9):e45376. doi: 10.1371/journal.pone.0045376.

Karki HS, Barphagha IK, Ham JH (2012)

A conserved two-component regulatory system, PidS/PidR, globally regulates pigmentation and virulence-related phenotypes of Burkholderia glumae. Mol Plant Pathol 13: 785-94.

Job History

2014 – 2015 Post-doctoral researcher, Purdue University, USA

2010 – 2013 Graduate research assistant (PhD), Louisiana State University, USA

2008 – 2010 Graduate research assistant (M.S), Louisiana State University, USA

2003 – 2008 Agriculture officer/Local adviser, Sarlahi and Chitwan, Nepal