Plasmid List

The SLJ series of plasmid constructs were created at the Sainsbury lab, and are publicly available.
Enquiries should be addressed to Prof. Jonathan Jones.


An extensive list of previous SLJ plasmids is available in the following reference:
Jones, J., D. G. Jones, L. Shulumukov, F. Carland, J. Engllish, S. R. Scofield, G. J. Bishop, and K. Harrison (1992). "Effective vectors for transformation, expression of heterologous genes, and assaying transposon excision in transgenic plants", Transgenic Research 1:285-297.

Enquiries regarding the availability of pJJ plasmids should be addressed to DNA Plant Technology Corporation.

The Structure of pJJ1881.

This shows how the T-DNA carrying sequences of pAGS111 were cloned into the Eco R1 site of pRK290 to make the binary vector plasmid from which all other SLJ binaries were ultimately derived.

Apa I and Nco I sites are also found in pRK290, and so cannot be used for cloning with these vectors, though they may prove useful as a diagnostic test for screening plasmids.

HIIIK signifies a Hin dIII site filled in with Klenow polymerase and dNTPs;

RIK indicates and Eco R1 sire similarly treated;

LB signifies T-DNA left border repeat sequence;

RB signifies the right border repeat;

nos indicates the nopaline synthase promoter;

ocs 3' indicates octopine synthase 3' end;

NPT indicates neomycin phosphotransferase;

tet indicates the pRK290 tetracycline resistance gene;

Gene transcriptional orientations are indiated by an arrow.

SLJ755I5

SLJ755I6

SLJ7292

SLJ7291

SLJ6991

NB! extra HindIII site not shown here may have been reconstituted when the polylinker was ligated in.

SLJ6999