Pre-doctoral Intern in the Krasileva Group
Closing Date: 05.01.2018 Reference ID: KK01/2018 Salary: £16,654 per annum (pro rata)
Predoctoral internships are aimed at individuals who want to gain practical experience in advance of starting a PhD. An internship is where an individual works to strengthen their professional experience before embarking on a PhD. Interns will be trained in a wide variety of techniques and skills, including but not limited to molecular biology, plant pathology, plant biology, bioinformatics, biochemistry/proteomics, and cell biology. Candidates are expected to have a BSc or MSc degree and are looking at this as an experience to enrich their training and experience prior to entering a PhD program. Predoctoral interns are not guaranteed to enter the PhD programme but the experience should strengthen their application for PhD studies at TSL or elsewhere. At the end of the internship, the Group Leader will conduct a formal performance review and a reference letter will be provided accordingly.
Our comparative genomics analysis of plant immune genes across flowering plants, including crops, highlighted candidate genes with distinct evolutionary patterns across both monocot and dicot species. To test the hypothesis that these genes are involved in disease resistance, we have devised a project to validate their role in disease resistance using the model plant species, Arabidopsis thaliana. The project will include genotyping of multiple A. thaliana T-DNA mutants using PCR, phenotyping mutants in a range of conditions including with pathogens, cloning and agro-infiltration among other skills. Confirmed functional genes will then be targeted for CRISPR/Cas9 gene editing in both Arabidopsis and crop species, such as wheat and tomato.
Students will be encouraged and supported to expand the project using their own initiative following discussion with mentors in the group. The expected involvement in current group’s research is summarized below. An experienced candidate will be encouraged to enhance research in the group with their previously acquired techniques.
- Genotyping mutant Arabidopsis lines using PCR
- Crossing Arabidopsis lines
- Performing pathogen inoculations and other disease resistance assays
- Observing developmental and/or conditional phenotypes of Arabidopsis T-DNA mutants
- Molecular cloning of plant immunity genes for Agroinfiltrations to observe immune response in Nicotiana benthamiana system
- CRISPR/Cas9 design
- Data analyses and contributions to manuscript preparation
- Contributing to scientific discussions within the group
- Interactions with other groups at TSL and Earlham and a wider scientific community
The duration of the internship is initially for 6 months.
Salary will be within the UEA Technical Staff Grade at £16,654 per annum (pro-rata)
Applicants should provide a CV, including the names and contact details of two referees, and a covering letter. Applications may be submitted online using the ‘Apply’ button below. Alternatively applications may be sent by e-mail to HR@tsl.ac.uk or by post to HR Manager, The Sainsbury Laboratory, Norwich Research Park, Norwich NR4 7UH, UK.
Informal enquiries should be directed to Ksenia.Krasileva@earlham.ac.ukApply