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Tissue Culture & Transformation

We provide a valuable support service to the research groups in The Sainsbury Laboratory. We generate large numbers of transgenic plants in a wide variety of species and cultivars for both model plants and crops and are active in researching new techniques for the generation of previously un-transformed plants species and cultivars.  We maintain germplasm collections, mostly for Solanum spp. and maintain and generate other research tools such as plant cell suspension cultures.

We also ensure provision of media, washed and sterilised laboratory consumables and processed seed.

Latest Publications

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Paulus JK, Kourelis J, Ramasubramanian S, Homma F, Godson A, Hörger AC, Hong TN, Krahn D, Carballo LO, Wang S, Win J, Smoker M, Kamoun S, Dong S, van der Hoorn RAL (2020)

Extracellular proteolytic cascade in tomato activates immune protease Rcr3. PNAS first published July 2, 2020 https://doi.org/10.1073/pnas.1921101117

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Pfeilmeier S, George J, Morel A, Roy S, Smoker M, Stransfeld L, Downie JA, Peeters N, Malone JG, Zipfel C (2018)

Expression of the Arabidopsis thaliana immune receptor EFR in Medicago truncatula reduces infection by a root pathogenic bacterium, but not nitrogen-fixing rhizobial symbiosis. Plant Biotechnol J. 2018 Aug 18. doi: 10.1111/pbi.12999.

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Tomlinson L, Yang Y, Emenecker R, Smoker M, Taylor J, Perkins S, Smith J, MacLean D, Olszewski NE, Jones JDG (2018)

Using CRISPR/Cas9 genome editing in tomato to create a gibberellin-responsive dominant dwarf DELLA allele. Plant Biotechnol J. 2018 May 24. doi: 10.1111/pbi.12952.

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Boschi F, Schvartzman C, Murchio S, Ferreira V, Siri MI, Galván GA, Smoker M, Stransfeld L, Zipfel C, Vilaró FL, Dalla-Rizza M (2017)

Enhanced Bacterial Wilt Resistance in Potato Through Expression of Arabidopsis EFR and Introgression of Quantitative Resistance from Solanum commersonii. Front. Plant Sci., 25 September 2017 | https://doi.org/10.3389/fpls.2017.01642

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