The availability of genome sequences has revolutionized biology and in particular proteomics.
With the increasing speed and resolving power of the latest mass spectrometers, comprehensive proteome coverage has become a (not to distant) reality. However mapping the increasing numbers of post-translational modifications on the proteome and identifying protein-protein interactions in the context of host microbe interactions remains a formidable challenge that keeps us from truly understanding signal transduction and plant immunity from a network biology perspective.
The proteomics team at TSL addresses this challenge through collaborative projects using our new discovery proteomics platform, the Orbitrap Fusion from Thermo Fisher, and applying targeted proteomics approaches on our Xevo TSQ triple quadrupole (Waters).
Petre B, Contreras MP, Bozkurt TO, Schattat MH, Sklenar J, Schornack S, Abd-El-Haliem A, Castells-Graells R, Lozano-Duran R, Dagdas YF, Menke FLH, Jones AME, Vossen JH, Robatzek S, Kamoun S, Win J (2021)
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. The Plant Cell, 2021;, koab069, https://doi.org/10.1093/plcell/koab069Read more
Janda M, Ludwig C, Rybak K, Meng C, Stigliano E, Botzenhardt L, Szulc B, Sklenar J, Menke FLH, Malone JG, Brachmann A, Klingl A, Robatzek S (2021)
Biophysical and proteomic analyses suggest functions of Pseudomonas syringae pv tomato DC3000 extracellular vesicles in bacterial growth during plant infection. bioRxiv preprint Feb 03, 2021 doi: https://doi.org/10.1101/2021.02.08.430144Read more
Derevnina L, Contreras MP, Adachi H, Upson J, Cruces AV, Xie R, Sklenar J, Menke FLH, Mugford ST, MacLean D, Ma W, Hogenhout SA, Goverse A, Maqbool A, Wu CH, Kamoun S (2021)
Plant pathogens convergently evolved to counteract redundant nodes of an NLR immune receptor network. bioRxiv preprint Feb 03, 2021 doi: https://doi.org/10.1101/2021.02.03.429184Read more
Abel NB, Buschle CA, Hernandez-Ryes C, Burkart SS, Deroubaix AF, Mergner J, Gronnier J, Jarsch IK, Folgmann J, Braun KH, Bayer E, Germain V, Derbyshire P, Menke FLH, Kemmerling B, Zipfel C, Küster B, Mongrand S, Marín M, Ott T (2021)
A hetero-oligomeric remorin-receptor complex regulates plant development. bioRxiv preprint Jan 29, 2021 doi: https://doi.org/10.1101/2021.01.28.428596Read more