The availability of genome sequences has revolutionized biology and in particular proteomics.
With the increasing speed and resolving power of the latest mass spectrometers, comprehensive proteome coverage has become a (not to distant) reality. However mapping the increasing numbers of post-translational modifications on the proteome and identifying protein-protein interactions in the context of host microbe interactions remains a formidable challenge that keeps us from truly understanding signal transduction and plant immunity from a network biology perspective.
The proteomics team at TSL addresses this challenge through collaborative projects using our new discovery proteomics platform, the Orbitrap Fusion from Thermo Fisher, and applying targeted proteomics approaches on our Xevo TSQ triple quadrupole (Waters).
Grubb L, Derbyshire P, Dunning K, Zipfel C, Menke FLH, Monaghan J (2020)
Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings. bioRxiv preprint Sept 17, 2020 doi: https://doi.org/10.1101/2020.09.16.299883Read more
Thor K, Jiang S, Michard E, George J, Scherzer S, Huang S, Dindas J, Derbyshire P, Leitão N, DeFalco TA, Köster P, Hunter K, Kimura S, Gronnier J, Stransfeld L, Kadota Y, Bücherl CA, Charpentier M, Wrzaczek M, MacLean D, Oldroyd GED, Menke FLH, M. Roelfsema MRG, Hedrich R, Feijó J, Zipfel C (2020)
The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature. 25 August 2020. DOI: 10.1038/s41586-020-2702-1Read more
Guo H, Ahn HK, Sklenar J, Huang J, Ma Y, Ding P, Menke FLH, Jones JDG (2020)
Phosphorylation-Regulated Activation of the Arabidopsis RRS1-R/RPS4 Immune Receptor Complex Reveals Two Distinct Effector Recognition Mechanisms. Cell Host Microbe. 2020 Mar 29. pii: S1931-3128(20)30172-4. doi: 10.1016/j.chom.2020.03.008.Read more
Ryder LS, Dagdas YF, Kershaw MJ, Venkataraman C, Madzvamuse A, Yan, X Cruz-Mireles N, Soanes DM, Oses-Ruiz M, Styles V, Sklenar J, Menke FLH, Talbot NJ (2019)
A sensor kinase controls turgor-driven plant infection by the rice blast fungus. Nature 09 Oct 2019. doi: https://doi.org/10.1038/s41586-019-1637-xRead more