The availability of genome sequences has revolutionized biology and in particular proteomics.
With the increasing speed and resolving power of the latest mass spectrometers, comprehensive proteome coverage has become a (not to distant) reality. However mapping the increasing numbers of post-translational modifications on the proteome and identifying protein-protein interactions in the context of host microbe interactions remains a formidable challenge that keeps us from truly understanding signal transduction and plant immunity from a network biology perspective.
The proteomics team at TSL addresses this challenge through collaborative projects using our new discovery proteomics platform, the Orbitrap Fusion from Thermo Fisher, and applying targeted proteomics approaches on our Xevo TSQ triple quadrupole (Waters).
Hegenauer V, Slaby P, Körner M, Bruckmüller JA, Burggraf R, Albert I, Kaiser B, Löffelhardt B, Droste-Borel I, Sklenar J, Menke FLH, Maček B, Ranjan A, Sinha N, Nürnberger T, Felix G, Krause K, Stahl M, Albert M (2020)
The tomato receptor CuRe1 senses a cell wall protein to identify Cuscuta as a pathogen. Nat Commun. 2020 Oct 20;11(1):5299. doi: 10.1038/s41467-020-19147-4.Read more
Petre B, Contreras MP, Bozkurt TO, Schattat MH, Sklenar J, Schornack S, Abd-El-Haliem A, Castells-Graells R, Lozano-Duran R, Dagdas YF, Menke FLH, Jones AME, Vossen JH, Robatzek S, Kamoun S, Win J (2020)
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. bioRxiv preprint Sept 24, 2020 doi: https://doi.org/10.1101/2020.09.24.308585Read more
Grubb L, Derbyshire P, Dunning K, Zipfel C, Menke FLH, Monaghan J (2020)
Large-scale identification of ubiquitination sites on membrane-associated proteins in Arabidopsis thaliana seedlings. bioRxiv preprint Sept 17, 2020 doi: https://doi.org/10.1101/2020.09.16.299883Read more
Thor K, Jiang S, Michard E, George J, Scherzer S, Huang S, Dindas J, Derbyshire P, Leitão N, DeFalco TA, Köster P, Hunter K, Kimura S, Gronnier J, Stransfeld L, Kadota Y, Bücherl CA, Charpentier M, Wrzaczek M, MacLean D, Oldroyd GED, Menke FLH, M. Roelfsema MRG, Hedrich R, Feijó J, Zipfel C (2020)
The calcium-permeable channel OSCA1.3 regulates plant stomatal immunity. Nature. 25 August 2020. DOI: 10.1038/s41586-020-2702-1Read more