The availability of genome sequences has revolutionized biology and in particular proteomics.
With the increasing speed and resolving power of the latest mass spectrometers, comprehensive proteome coverage has become a (not to distant) reality. However mapping the increasing numbers of post-translational modifications on the proteome and identifying protein-protein interactions in the context of host microbe interactions remains a formidable challenge that keeps us from truly understanding signal transduction and plant immunity from a network biology perspective.
The proteomics team at TSL addresses this challenge through collaborative projects using our new discovery proteomics platform, the Orbitrap Fusion from Thermo Fisher, and applying targeted proteomics approaches on our Xevo TSQ triple quadrupole (Waters).
Goto Y, Maki N, Sklenar J, Derbyshire P, Menke FLH, Zipfel C, Kadota Y, Shirasu K (2020)
The phagocytosis oxidase/Bem1p (PB1) domain-containing protein PB1CP negatively regulates the NADPH oxidase RBOHD in plant immunity. bioRxiv preprint Dec 29, 2020 doi: https://doi.org/10.1101/2020.12.28.423414Read more
Sun X, Lapin D, Feehan JM, Stolze SC, Kramer K, Dongus JA, Rzemieniewski J, Blanvillain-Baufume S, Harzen A, Bautor J, Derbyshire P, Menke FLH, Finkemeier I, Nakagami H, Jones JDG, Parker JE (2020)
Pathogen effector recognition-dependent association of NRG1 with EDS1 and SAG101 in TNL receptor immunity. bioRxiv preprint Dec 22, 2020 doi: https://doi.org/10.1101/2020.12.21.423810Read more
Hegenauer V, Slaby P, Körner M, Bruckmüller JA, Burggraf R, Albert I, Kaiser B, Löffelhardt B, Droste-Borel I, Sklenar J, Menke FLH, Maček B, Ranjan A, Sinha N, Nürnberger T, Felix G, Krause K, Stahl M, Albert M (2020)
The tomato receptor CuRe1 senses a cell wall protein to identify Cuscuta as a pathogen. Nat Commun. 2020 Oct 20;11(1):5299. doi: 10.1038/s41467-020-19147-4.Read more
Petre B, Contreras MP, Bozkurt TO, Schattat MH, Sklenar J, Schornack S, Abd-El-Haliem A, Castells-Graells R, Lozano-Duran R, Dagdas YF, Menke FLH, Jones AME, Vossen JH, Robatzek S, Kamoun S, Win J (2020)
Host-interactor screens of Phytophthora infestans RXLR proteins reveal vesicle trafficking as a major effector-targeted process. bioRxiv preprint Sept 24, 2020 doi: https://doi.org/10.1101/2020.09.24.308585Read more